Effect of Vitamin D3 Supplementation on Symptomatic Uterine Fibroid in Women With Vitamin D Deficiency

Inclusion Criteria:

.Women aged between 18-50 not currently pregnant, lactating, or planning to conceive during the study period.

• Women with a confirmed diagnosis of uterine fibroids (leiomyoma) through ultrasound.

• Serum 25-hydroxyvitamin D [25(OH)D] levels below 20 ng/mL (indicating vitamin Ddeficiency).

• Presence of symptoms associated with uterine fibroid, such as menorrhagia (heavymenstrual bleeding), or bulk-related symptoms (e.g., urinary frequency).

• Participants should be generally healthy, with no history of other chronic illnessesthat could affect the study's outcomes (e.g., autoimmune diseases, cancers, severeliver or kidney diseases).

• Willingness to provide written informed consent for participation in the study.

• No current use of hormonal therapy, other vitamin D supplements, or medicationsknown to influence calcium metabolism or uterine fibroid growth (e.g., GnRHagonists, anti-estrogens).

• Participants must be willing and able to adhere to the study protocol, includingattending follow-up visits and completing any required assessments or tests.

Exclusion Criteria:

• Women younger than 18 or older than 50 years.

• Serum 25-hydroxyvitamin D [25(OH)D] levels of 20 ng/mL or higher.

• Women without a confirmed diagnosis of uterine fibroids (leiomyoma) through imaging.

• Pregnant or breastfeeding women, or those planning to conceive during the studyperiod.

• Presence of chronic illnesses such as autoimmune diseases, cancers, or severe liveror kidney disease that could interfere with the study's outcomes.

• Current or recent use of hormonal therapy, other vitamin D supplements, ormedications that affect calcium metabolism or uterine fibroid growth (e.g., GnRHagonists, anti-estrogens).

• Women who have undergone surgical interventions (e.g., myomectomy, hysterectomy) orother medical treatments specifically for uterine fibroid within the past 6 months.

• Women with conditions like hyperparathyroidism or calcium disorders that mightaffect vitamin D metabolism or fibroid growth.