Comparison of IL-8, FGF-1 and VEGF Levels in Pulpal Tissue, GCF and Dentinal Fluid Samples of Healthy, Reversible and Irreversible Pulpitis Diagnosed Teeth

Last updated: April 19, 2025
Sponsor: Kırıkkale University
Overall Status: Active - Recruiting

Phase

N/A

Condition

Acute And Chronic Dental Pain

Inflammation

Treatment

N/A

Clinical Study ID

NCT06936553
2024/149
  • Ages 14-60
  • All Genders
  • Accepts Healthy Volunteers

Study Summary

This study will include 50 patients diagnosed with healthy, reversible and irreversible pulpitis. IL-8, FGF-1 and VEGF levels will be compared using pulpal tissue, gingival crevicular fluid, dentinal fluid samples collected from the teeth of these patients. First, the DOS sample will be collected non-invasively using periopaper strips without local anesthesia. Then, the caries will be cleaned by applying local anesthesia and rubber dam to the patient and a dentinal fluid sample will be collected noninvasively using PVDF membranes. In teeth with pulp exposure due to caries, the pulp tissue that needs to be removed will not be disposed of but collected as a sample. Routine root canal treatment procedures will then continue and treatment of the teeth will be completed. The collected samples will be analyzed for IL-8, FGF-1 and VEGF levels by ELISA test.

Eligibility Criteria

Inclusion

Inclusion Criteria:

Control group:

  • Unrestored, caries-free vital teeth with healthy pulp

  • Orthodontically indicated teeth for extraction

  • wisdom teeth indicated for extraction in terms of oral hygiene

  • teeth that require root canal treatment for prosthetic purposes

Reversible pulpitis group:

  • Teeth that react mildly to cold, hot, sour or sweet stimuli

  • Teeth with no history of spontaneous pain

  • Teeth that respond to cold test and electric pulp test within normal limits

  • Teeth that are not sensitive to chewing or percussion

Irreversible pulpitis group:

  • Teeth with a history of continuous moderate or severe spontaneous pain

  • Teeth sensitive to percussion

  • Teeth without periapical pathology

Exclusion

Exclusion Criteria:

  • Patients requiring antibiotic prophylaxis.

  • Patients with diabetes or haematological diseases.

  • Patients who have used antibiotics or anti-inflammatory drugs in the last threeweeks.

  • Patients who are pregnant during treatment.

  • Patients with intense plaque and tartar on their teeth, redness and bleeding in thegums.

  • Patients with severe gingivitis, generalised periodontitis or periodontal pocketdepth of more than 3 mm.

  • Patients with no response to pulp sensitivity tests, presence of sinus tract,presence of periapical pathology on radiography.

  • Teeth without pulp exposure after caries removal

Study Design

Total Participants: 50
Study Start date:
March 03, 2025
Estimated Completion Date:
July 03, 2025

Connect with a study center

  • Kırıkkale University Faculty of Dentistry

    Kırıkkale, 71450
    Turkey

    Active - Recruiting

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