Application of CfDNA Methylation Detection in Auxiliary Diagnosis of Breast Cancer

Inclusion Criteria for All the Participants:

• With confirmed pathological diagnosis and molecular subtyping results

• Ability to provide a written informed consent

Exclusion Criteria for Cancer Arm Participants:

• Patients with a history of or currently suffering from other malignancies

• Pregnant or planning to become pregnant female patients

• Patients who have received cancer treatment, including surgery, chemotherapy, radiotherapy, targeted therapy, and immunotherapy, before blood draw

• Patients with a history of blood transfusion within the past month

• Patients with a known history of allogeneic organ transplantation and allogeneic hematopoietic stem cell transplantation

• Patients with persistent fever or undergoing anti-inflammatory treatment within 14 days before blood draw

• Any other conditions that the researcher deems may make the patient unsuitable for inclusion in the study or may interfere with the completion of the study

• Patients in poor physical condition who are not suitable for blood draw

• Patients who cannot provide informed consent or refuse blood draw

Exclusion Criteria for Benign Diseases Arm Participants:

• History of malignancies

• Current malignancies or precancerous lesions