The NUTRIOME Study - a Data-driven Precision Nutrition Intervention

Inclusion Criteria:

• Men and women

• Age 40 to 70 years

• Body mass index (BMI) 27-35 kg/m2

• Signed informed consent

Exclusion Criteria:

• History of diagnosed cardiovascular disease (e.g. stroke, heart disease)

• Diagnosed type 1 or type 2 diabetes

• Finger prick glucose test at screening indicating diabetes (fasting >6.9 mmol/L;non-fasting >11.0 mmol/L)

• Antibiotic use in the last 3 months

• Taking medication that can interfere with the planned outcome measures, as judged bythe study physician (e.g. peroxisome proliferator-activated receptor-α [PPAR-α] orPPAR-γ agonists [fibrates], sulfonylureas, biguanides, α-glucosidase inhibitors,thiazolidinediones, repaglinide, nateglinide and insulin, chronic use of NSAIDs).Statins and hypertension medication are allowed if the medication regimen has beenstable for the last three months

• Stomach and gastrointestinal conditions (e.g. Morbus Chron, Ulcerative colitis,irritable bowel syndrome, malabsorption, colostomy, bowel resection, gastric bypasssurgery etc.)

• History of major gastrointestinal surgery

• Anaemia: Hb concentrations < 8.5 mmol/L for men and < 7.5 mmol/L for women

• Recent blood donation (within 2 months prior to the screening)

• Recent participation in another intervention study (within 2 months prior to thescreening)

• Allergy or intolerance to medical skin adhesives

• Chronic or acute inflammatory conditions (e.g., rheumatoid arthritis, psoriaticarthritis)

• Autoimmune disorders

• Thyroid disorders

• Significant renal or liver dysfunction or chronic kidney or liver disease

• Known food allergies/intolerances to intervention foods or food products used in thestudy

• Following specific dietary regimen that could impact results or not suitable for thestudy design (e.g. vegetarian, vegan)

• Habitual tobacco consumption (e.g. smoking, use of snus, vaping)

• Alcohol intake ≥ 14 alcoholic beverages/week for women and ≥ 21 alcoholicbeverages/week for men

• Current or planned pregnancy or lactating

• Other serious medical conditions that could interfere with participation or studyoutcomes

• Unable to sufficiently understand written and spoken national language (where thestudy centre is located) to provide written consent and understand information andinstructions from the study personnel.

• Deemed unsuitable for participation in the trial, for any reason, as judged by theresearch physician or PI.

• Working at the division conducting the trial at any of the three study centres.

• Do not have a mobile phone that is suitable for the use of a research app.