Peptide Vaccine for Fibrolamellar Hepatocellular Carcinoma Patients and Other Tumor Entities Carrying the Driver Fusion DNAJB1-PRKACA

Inclusion Criteria:

• Ability to understand and willingness to sign a written informed consent document.

• Histologically confirmed Fibrolamellar hepatocellular carcinoma (FL-HCC) or othermalignant disease in an adjuvant setting, defined as:

• Presence of DNAJB1-PRKACA fusion transcript, assessed by RNA-based NGS orRT-PCR

• Achievement of complete remission (CR) according to RECIST1.1 by any of thefollowing therapeutic measures:

• surgical procedures,

• radiotherapy,

• local therapeutic measures (e.g. TACE, SIRT, etc.)

• systemic treatment (e.g. chemotherapy)

• Age ≥ 12 years. Note: Subjects aged ≥ 12 years but < 18 are eligible to enroll onlyafter 6 adult patients have been enrolled in the study.

• Eastern Cooperative Oncology Group (ECOG) performance status ≤ 2.

• Adequate laboratory values for

• Absolute Lymphocyte Count > 500 /µl

• Platelets > 50.000 /µl

• Creatinine clearance GFR > 30 ml/min

• Alanine aminotransferase (ALT) and aminotransferase (AST) ≤ 5 times upper limitrange

• Bilirubin ≤ 3 mg/dl

• Negative serological hepatitis B test or negative PCR in case of positiveserological test without evidence of an active infection, negative testing ofhepatitis C RNA, negative HIV test within 6 weeks prior to study inclusion.

• Female patients of child bearing potential (FCBP) and male patients with partners ofchild bearing potential who are sexually active must agree to the use of twoeffective forms (at least one highly effective method) of contraception. This shouldbe started from the signing of the informed consent and be continued until 3 months (both female and male patients) after last dose of the vaccination

• For FCBP two negative pregnancy tests (sensitivity of at least 25 mIU/mL) prior tofirst application of the study drug (vaccination at visit V1), one at screening andthe other one at visit V1 prior (< 24h) to first vaccination.

• Postmenopausal or evidence of non-child-bearing status.

• Be willing to minimize blood and body fluid exposure after vaccination until end ofstudy

• Refrain from sperm or ovary egg donation

• Refrain from blood donation

Exclusion Criteria:

• Pregnant or breastfeeding.

• Unwilling or unable to follow the study schedule for any reason.

• Concurrent or previous treatment within 14 days in another interventional clinicaltrial with an investigational anti-cancer treatment.

• Concurrent treatment with any of the following therapeutic measures:

• surgical procedures,

• radiotherapy,

• local therapeutic measures (e.g. TACE, SIRT, etc.)

• systemic treatment (e.g. chemotherapy)

• Concurrent or previous treatment within 6 months with an anti-cancer vaccinetreatment.

• Any live vaccine therapy used for prevention of infectious diseases within 28 daysof study treatment

• Known sensitivity to or history of allergic reactions to investigational drug.

• Active autoimmune disease that has required systemic treatment in the past 2 years,or a documented history of clinically severe autoimmune disease, or a syndrome thatrequires systemic steroids (> 10 mg per day) or immunosuppressive agents (Pleasenote, patients after liver transplantation requiring immunosupressants are allowed).

• Uncontrolled intercurrent illness including, but not limited to, uncontrolledinfection, symptomatic congestive heart failure, unstable angina, cardiacarrhythmia, metastatic cancer, or psychiatric illness/social situations that wouldlimit compliance with study requirements.