Characterization of Human Immune Signatures to Zoonotic Virus Exposure in Cambodia

Inclusion Criteria:

1. Capacity to provide informed consent.

2. Adult aged 18-65 years.

3. Have interaction with suspected infected animals within the last 2 years, including (but not limited to) the following risk factors:

4. Hunting, slaughtering, or consuming suspected infected animals;

5. Fruit collection, date palm sap harvesting, or tree pruning within agriculturalplantations containing bat roosts;

6. Bat guano farming;

7. Ancillary work in live animal markets or wild animal habitats identified aslikely containing infected animals (e.g., provision of cleaning,transportation, or tourism services);

8. Living within 5 km of identified animal markets or wild animal habitatsidentified as likely containing infected animals.

9. Willing to allow biological samples and data to be stored for future research.

Exclusion Criteria:

1. Pregnancy (based on self-reporting).

2. Any underlying, chronic, or current medical condition that, in the opinion of theinvestigator, would interfere with participation in the study (e.g., inability orgreat difficulty in drawing blood, known anemia).

3. Self-reported symptoms suggestive of acute infection (acute myalgias, arthralgias,headache, retro-orbital pain, dyspnea, rash) within 7 days prior to enrollment.

4. Signs suggestive of acute infection (fever, defined as internal temperature >38°C;hypoxemia, defined as peripheral oxygen saturation of <90%; hypotension, defined assystolic blood pressure <90 mm Hg or diastolic blood pressure <50 mm Hg) present atscreening.

5. Self-reported diagnosis of immune deficiency, including HIV infection, chroniccorticosteroid use (≥10 mg prednisone dose or its equivalent for a continuous periodof ≥30 days within the last 1 year), ongoing or prior (within the last 10 years)receipt of chemotherapy or immunotherapy, or current hematological malignancy.

6. Receipt of blood products, including immunoglobulin products, within 120 days ofstudy enrollment.