Application of Platelet-rich Plasma (PRP) in Reproductive Medicine

Inclusion Criteria:

• Premature ovarian insufficiency: ① Women aged >18 and <40 years old; ②Oligomenorrhea or amenorrhea for more than 4 months (excluding pregnancy); ③ Basalfollicle stimulating hormone (FSH) >25IU/L (interval >4 weeks) at least 2 times, ortotal antral follicles (AFC) <5, or anti-Mullerian hormone (AMH) <1.1ng/mL.

• Poor ovarian response: ① Advanced age (≥40 years) or other risk factors for poorovarian response; ② Poor ovarian response in previous IVF cycle, with ≤3 oocytesretrieved using conventional protocols; ③ Decreased ovarian reserve (antral folliclecount <57 or anti-Müllerian hormone <0.51.1 ug/L). Meeting any 2 of the above 3criteria is sufficient.

• Intrauterine adhesion: ① Diagnosed by hysteroscopy and scored ≥5 points according tothe American Fertility Society criteria (1988); ② With symptoms such as reducedmenstrual flow amenorrhea, periodic lower abdominal pain, infertility, or recurrentmiscarriage; ③ Adult women with the desire to conceive.

• Thin endometium: ①Endometrial thickness <7mm on ovulation day or on the day of humanchorionic gonadotropin (HCG) injection, or <7mm on progesterone conversion day whenusing conventional hormone replacement therapy, ②adult women with the desire tocomceive.

• Repeated implantation failure: Women under 40 years old who have failed to achieveclinical pregnancy after transplanting at least three high-quality embryos withinthree fresh or frozen cycles. High-quality embryos include: Day 3 embryos (with ≥8cells, evenly sized blastomeres, and <10% fragmentation) and blastocysts (≥3BB).

Exclusion Criteria:

• Alanine aminotransferase > 3 times the normal upper limit or estimated glomerularfiltration rate (EGFR) < 90 ml/min;

• Suffering from hemorrhagic diseases (such as hemophilia) or taking anticoagulant orantiplatelet drugs;

• Suffering from serious mental illness, cardiovascular and cerebrovascular diseasesand malignant tumors;

• Suffering from diabetes with poor blood sugar control or other systemic diseases;

• Drug abuse, alcoholism or drug addiction;

• Participated in other interventional clinical studies within half a year;

• Poor compliance;

• Other circumstances that are not suitable for participating in the study.