The EPIC Study: Exploring Paternal Age and the Influence on Blastocyst Culture

Inclusion Criteria:

• Undergoing first IVF cycle

• Electing single embryo transfer

• Electing PGT-A of their embryos

• Female partners age <42 years old at start of VOR cycle, but >18 years old.

• AMH ≥ 1.2 ng/mL

• AFC ≥ 8

• FSH ≤ 12IU/L

• At least 4 mature oocytes (M2s) retrieved at the VOR procedure in order to randomize

• Intention to transfer the morphological best quality, euploid, embryo at the frozenembryo transfer procedure

Exclusion Criteria:

• Contraindication to IVF

• Clinical indication for preimplantation genetic testing (i.e., screening for singlegene disorder, chromosomal translocation, or any other disorders requiring a moredetailed embryo genetic analysis)

• Male partner with azoospermia or oligozoospermia (<500,000 total motile spermatozoaon the most recent semen analysis within one year of enrollment)

• Planned for previously cryopreserved sperm to be used for ICSI

• Donor sperm

• Male partner with Y-chromosome microdeletion

• Male partner with any Karyotype other than 46,XY

• Male partner requiring surgically obtained sperm either via testicular or epididymalretrieval procedures

• Uncorrected hydrosalpinges that communicate with the endometrial cavity

• Endometrial Insufficiency, as defined by a prior cycle with maximal endometrialthickness <6mm,), or persistent endometrial fluid

• Donor oocyte or embryo cycles

• Gestational carriers