Exploratory Research on Constructing a Computational Biological Model Based on NGS for MRD After Breast Cancer Surgery

Inclusion Criteria:

1. Age ≥ 18 years old, gender not limited; 2. Obtain plasma samples from thesubjects during a 3-year follow-up period; 3. The subjects fully understand thestudy and voluntarily sign the informed consent form; 4. Able to cooperate witha 3-year follow-up visit to the hospital; 5. The physical fitness status score (ZPS) of the ECOG scoring criteria for the subjects must be ≤ 1; 6. Lifeexpectancy ≥ 5 years; 7. Subjects who meet the following criteria:

2. Histopathology confirmed primary breast cancer (unlimited molecular type);

3. Radical breast cancer resection is expected;

4. Adopting postoperative adjuvant therapy or preoperative neoadjuvant therapy;

Exclusion Criteria:

1. The subject is pregnant or breastfeeding; 2. Serious mental illness or drugabuse; 3. Unable to obtain the subject's plasma during this period; 4. Ifthe subject has a non primary malignant tumor of the breast with a clearpathological diagnosis within 5 years before enrollment: 5. If the subject hassuspected non breast malignant tumors (such as B-ultrasound, CT, etc.) onimaging within the past year of enrollment, but without pathologicalconfirmation; 6. Clinical suspicion of distant metastatic lesions; 7. Thesubject has received any blood product infusion therapy within the past 30days; 8. Known carriers of pathogenic genetic mutations;