Cryopreservation Medium With Apigenin on Post-thaw Human Sperm

Last updated: March 16, 2025
Sponsor: Chulalongkorn University
Overall Status: Completed

Phase

N/A

Condition

N/A

Treatment

Apigenin

Clinical Study ID

NCT06560216
062267
  • Ages 18-45
  • Male
  • Accepts Healthy Volunteers

Study Summary

The goal of this clinical trial is to compare the effect of apigenin supplementation in freezing medium with no apigenin supplementation in freezing medium on post-thaw human sperm motility. It will also evaluate the sperm viability, total antioxidant capacity, and oxidative stress markers. The main questions it aims to answer are:

  • Does apigenin supplementation in freezing medium improve the post-thaw sperm motility?

  • Does apigenin supplementation in freezing medium improve the post-thaw sperm viability, total antioxidant capacity, and oxidative stress?

Eligibility Criteria

Inclusion

Inclusion Criteria:

  • Healthy men aged 18-45 years who can receive semen analysis services at theReproductive Biology Unit, Chulalongkorn Hospital, Thai Red Cross Society

  • Normal semen analysis results according to the World Health Organization criteria (Volume ≥ 1.4 mL, Sperm concentration ≥ 16 million/mL, Total motility ≥ 42%,Progressive motility ≥ 30%)

  • Giving consent

Exclusion

Exclusion Criteria:

  • A history of accidents, radiation to the testes, or a history of male reproductivesystem surgery

  • Undescended testis

  • Varicocele

  • A history of taking certain medications that affect sperm production, such aschemotherapy, hormones

  • Currently having sexually transmitted disease

Study Design

Total Participants: 57
Treatment Group(s): 1
Primary Treatment: Apigenin
Phase:
Study Start date:
October 28, 2024
Estimated Completion Date:
March 03, 2025

Study Description

All normal semen samples from participants who give written informed consent and meet the eligibility requirements will be included in the study. Prepared semen samples were divided into 3 groups.

  1. Pre-freezing sperm suspension

  2. Frozen-thawed control without apigenin supplementation in freezing medium

  3. Frozen-thawed sperm suspension with apigenin 0.2 mM supplementation in freezing medium Baseline sperm motility will be measured in pre-freezing sperm suspension. The last 2 groups will be stored in liquid nitrogen for at least one week. After thawing, the sperm suspensions will be evaluated for motility, viability, total antioxidant capacity, and oxidative stress markers.

Connect with a study center

  • Chulalongkorn Hospital

    Bangkok, 10330
    Thailand

    Site Not Available

  • Chulalongkorn University

    Bangkok, 10330
    Thailand

    Site Not Available

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