This study will be performed as a prospective, single arm study. Consecutive patients
eligible for a standard-of-care injection will be enrolled from the Utah Center for
Bleeding and Clotting Disorders. The University of Utah non-operative sports medicine
team currently performs hundreds of injections monthly, and the PI works closely with the
orthopedic complaints of patients with bleeding disorders. From a research standpoint,
the investigators have successfully performed numerous randomized controlled prospective
trials on a variety of musculoskeletal conditions in the past and have already validated
this method on a large cohort of patients with knee osteoarthritis (OA).
Potential subjects will then be approached by a member of the research team to discuss
the consideration of taking part in the study if they meet the inclusion criteria of this
study project. Using all forms of good clinical practice, a member of the study team will
review with the participant the Informed Consent Form, allow participant to read the
informed consent (ICF) and ask questions prior to signing the ICF. Potential subjects may
also be asked by other physicians or staff members familiar with the study.
Low Cost-Platelet Rich Plasma (LC-PRP) preparation method: See Documents for full
details. This method follows the same procedures as the standard-of-care, FDA-cleared
method used by numerous commercial machines; thus it does not require additional FDA
clearance or approval. To summarize the full method in the appendix, 45mL of blood is
drawn from the subject in a standard-of-care manner into 3 x 20mL syringes, each
containing 2mL of sodium citrate (anticoagulant). Each syringe is prepared and capped,
then placed into a centrifuge. The resulting separated blood is then drawn off into a
single new sterile syringe, which can be used for the injection. Blood and PRP analysis:
The investigators will perform a full analysis (a complete blood count (CBC) with
differential) on subjects' whole blood and their prepared LC-PRP. Importantly, the
investigators will include all data required in the PRP minimum reporting standards,
including "platelet, differential leukocyte, and red cell analysis of all samples." Also
note that although the investigators are collecting these values for the purposes of the
study, CBC values are not needed in the community.
Injections: A total of 20 joints (estimated 10-15 patients) will receive injections,
performed at day 0 and 3 weeks, for a total of two injections - two injections have
demonstrated superior outcomes to one, and will increase our number of injections to
improve the proof of feasibility. All subjects will receive ultrasound-guided
intra-articular joint injections by an experienced sports medicine provider after local
anesthesia with 1% lidocaine to the extra-articular structures, namely the skin and joint
capsule, to increase comfort. Note: though the investigators are using ultrasound
guidance to ensure that they are guaranteeing accurate injectate placement for this
study, this is not necessarily required in the community setting. These will be performed
after the patient's typical factor infusions, if applicable.
Questionnaire: The investigators will collect demographic information along with baseline
and subsequent functional patient reported outcomes, namely the WOMAC (knee), FAOS
(ankle), and the QuickDASH (elbow). REDCap will be used to administer surveys and store
data. The investigators will additionally ask about adverse events, pain, global
satisfaction, global injection satisfaction, and medications.
Data collection time points and follow-up: Follow-up data (see timeline below) will be
administered electronically. Subjects will be queried by email, text message, in person,
and/or by telephone regarding treatment outcomes at 0, 1, 3, and 6 months after the
second injection. Three months will be the primary endpoint, and six months will be an
exploratory, unfunded endpoint.
LC-PRP Outcomes: As this is a feasibility study, it is likely that this study will
be underpowered for clinical outcomes. However, the investigators will still monitor
pain and the above outcome measures and identify potential time-related
improvements.
Economic Evaluation: The investigators will track staff-, time-, equipment-, and
supply-related costs to accurately identify costs associated with each injection.
This will ensure that costs are accurately recorded.