Application of Autologous Adipose Stromal Vascular Fraction in the Treatment of Premature Ovarian Insufficiency

Inclusion Criteria:

• Women aged ≥20 and ≤39 years who have childbearing requirements.

• Cessation of menstruation or oligomenorrhea for at least 4 months

• Serum level of basal follicle stimulating hormone (FSH) >25 U/L at least 2 times (during the second to fourth day of the menstrual cycle or during amenorrhea; Theinterval between the two tests is 4 weeks)

• Serum level of Anti-Mullerian hormone (AMH) ≤1.1 ng/ml

• Women with intact uterus and bilateral adnexa.

• Voluntary participation and informed consent obtained.

Exclusion Criteria:

• Women with autoimmune diseases.

• Women with abnormal and uncontrolled thyroid function.

• Women with tumors in bilateral adnexa that are not clearly benign or malignant.

• Women with a history of malignant tumors, radiation therapy or chemotherapy.

• Women with a history of venous thrombosis or pulmonary embolism during the screeningperiod.

• Women with severe illnesses that are not suitable for pregnancy, such as diseases ofthe circulatory system, urinary system, digestive system, endocrine system,respiratory system, immune system, mental or neurological system, etc.

• Women who continuously used systemic glucocorticoids or other immunosuppressantsfor≥3 weeks within 6 months before administration and women who used systemicglucocorticoids (prednisone≥20 mg/d or equivalent dose) or other immunosuppressantswithin 3 days before administration.

• Women who are allergic to the active ingredients or excipients of test drugs.

• Women with a family history of severe genetic diseases or gynecologic malignancies.