Deep Phenotyping of Peripheral Blood Cells and Circulating Factors in Metabolic Diseases

Inclusion Criteria:

1. Age > 18 years old Additional inclusion criteria for case groups:
2. High risk group for significant liver fibrosis
3. FIB-4 score ≥ 1.3 AND 2. Fibroscan measurement ≥ 8kPa
4. Steatotic Liver Disease group
5. Diagnosis of steatosis in ultrasound AND CAP > 275 dB/m
6. Prediabetes
7. HbA1c >5.7 AND <6.5% OR/AND
8. Fasting Glucose 100-125 mg/dl OR/AND
9. Glucose at 120 min of OGTT between 140-200 mg/dl
10. Diabetes 1. HbA1c ≥ 6.5% OR/AND 2. Fasting Glucose > 126 mg/dl OR/AND 3. Glucose at 120 min of OGTT > 200 mg/dl If a subject does not fulfil the additional criteria for participating in a casegroup, then he/she will be included in the respective control group which will be: A#) Low risk for significant liver fibrosis 1. Fibroscan measurements < 8kPa B#) Nosteatosis group
11. No steatosis in liver ultrasound AND CAP ≤ 275 dB/m C#) Normal glucose tolerancetest group
12. HbA1c < 5.7% AND
13. Fasting glucose < 100 mg/dl AND
14. Glucose at 120 min of OGTT <140 mg/dl

Exclusion Criteria:

1. Diabetes mellitus Typ 1
2. BMI < 18.5 kg/m2
3. Transfusion of blood or major bleeding in the last six months
4. Anaemia with haemoglobin < 9,0 g/dl
5. Chronic alcohol or drug abuse
6. Presence of any acute or chronic liver disease apart from non-alcoholic fattyliver disease (i.e. viral, autoimmune or alcoholic hepatitis, haemochromatosis,Morbus Wilson etc.)
7. Systemic infections (CRP > 1 mg/dl)
8. Medications that affect blood glucose levels (e.g. antidiabetics [except from thesubjects forming the diabetes group], steroids) in the last six months
9. Medications that affect coagulation (e.g. anticoagulants and antiplatelet agents)in the last six months
10. Medications that affect immune function (e.g. immunosuppressive drugs) in thelast six months
11. Pregnancy or breastfeeding
12. Severe psychic disorders
13. Inability to follow the study protocol
14. Have any medical condition unsuitable for inclusion in the study, in the opinionof the investigator Additional exclusion criteria for MRI:
15. Pacemaker
16. Artificial heart valve
17. Metal prosthesis
18. Implanted magnetic metal parts
19. Spirals
20. Fixed metal dental braces
21. Acupuncture needle
22. Insulin pumps
23. By MRI > 3 Tesla: Tattoos, permanent eyeliner
24. Claustrophobia or any other condition, such as psychiatric disorder, that in theopinion of the investigator may prevent the participant from following andcompleting the protocol
25. Subject dimensions not allowing the performance of MRI