Open-label, Single Center, Single-arm, Phase 2 Study of Neoadjuvant Pembrolizumab in Combination With Carboplatin and Paclitaxel in Patients With Stage 1 cT1b-T1cN0M0 Triple Negative Breast Cancer

Inclusion Criteria:

Participants are eligible to be included in the study only if all of the following criteria apply:

1. Participants must have histologically confirmed ER negative, PR negative andHER2-negative (TNBC) defined as ER<10%, PR<10%, and HER2 negative (per 2018 ASCO CAPguidelines). All pathology will be confirmed at the MD Anderson Houston Campus.Participants with tumors designated as HER2 equivocal (per ASCO CAP guidelines) areeligible if in view of treating physician patient is not considered a candidate forHER2-targeted therapy. Participants with weakly ER or PR positive disease, definedas ER and/or PR between 1-9% by immunohistochemistry, are eligible if the treatingphysician considers the participants not eligible for adjuvant endocrine therapy.

2. AJCC 8 anatomic tumor Stage 1 T1b-T1c, N0, M0. All participants with clinicallysuspicious nodes must undergo core or fine needle biopsy per standard clinicalpractice to pathologically assess at least 1 suspicious index node. Participantswith suspicious nodes that are biopsy negative will be eligible.

3. Male/female participants who are at least 18 years of age on the day of signinginformed consent with histologically confirmed diagnosis of Stage 1 T1b-T1cN0M0 TNBCwill be enrolled in this study.

4. Male participants: A male participant must agree to use a contraception as detailedin Appendix 2 of this protocol during the treatment period and for at least 120days, corresponding to time needed to eliminate any study treatment(s) (e.g. 5terminal half-lives for pembrolizumab and/or any active comparator/combination) plusan additional 90 days (a spermatogenesis cycle) for study treatments with evidenceof genotoxicity at any dose after the last dose of study treatment and refrain fromdonating sperm during this period.

5. Female participants: A female participant is eligible to participate if she is notpregnant (see Appendix 2), not breastfeeding, and at least one of the followingconditions applies:

6. Not a woman of childbearing potential (WOCBP) as defined in Appendix 2 OR

7. A WOCBP who agrees to follow the contraceptive guidance in Appendix 2 duringthe treatment period and for at least 120 days (corresponding to time needed toeliminate any study treatment(s) (pembrolizumab and/or any activecomparator/combination) plus 30 days (a menstruation cycle) for studytreatments with risk of genotoxicity after the last dose of study treatment.

8. Participants who have AEs due to previous anticancer therapies must have recoveredto ≤Grade 1 or baseline. Participants with endocrine-related AEs who are adequatelytreated with hormone replacement or participants who have ≤Grade 2 neuropathy areeligible.

9. The participant provides written informed consent for the trial.

10. Archival tumor tissue sample or newly obtained [core, incisional or excisional]biopsy of a tumor lesion not previously irradiated has been provided.Formalin-fixed, paraffin embedded (FFPE) tissue blocks are preferred to slides.Newly obtained biopsies are preferred to archived tissue.

11. Have an Eastern Cooperative Oncology Group (ECOG) performance status of 0 to 1 (appendix 3). Evaluation of ECOG is to be performed within 7 days prior to the firstdose of study intervention.

12. Have adequate organ function as defined in the following table (Table 3). Specimensmust be collected within 10 days prior to the start of study intervention.

13. Non-English speaking participant can enroll in the study as long as they speak alanguage for which interpretation can be provided by a licensed interpreter eitherin person or virtually.

14. Criteria for known HIV positive participants.

15. Participants with HIV are eligible if they have well-controlled HIV withnegative viral load on ART and must not have had any AIDS-definingopportunistic infections within the past 12 months from initiation of C1D1study treatment.

16. HIV screening tests are not required unless: i. Known history of HIV ii. As mandated by local health authority

17. Criteria for known Hepatitis B and C positive participants Hepatitis B and Cscreening tests are not required unless:

• Known history of HBV or HCV infection

• As mandated by local health authority 13.1 Hepatitis B positive Participants

• Participants who are HBsAg positive are eligible if they have received HBVantiviral therapy for at least 4 weeks and have undetectable HBV viral loadprior to randomization.

• Participants should remain on anti-viral therapy throughout study interventionand follow local guidelines for HBV anti-viral therapy post completion of studyintervention. 13.2 Participants with history of HCV infection are eligible ifHCV viral load is undetectable at screening.

• Participants must have completed curative anti-viral therapy at least 4 weeksprior to randomization.

Table 3 Adequate Organ Function Laboratory Values System Laboratory Value Hematological Absolute neutrophil count (ANC) = ≥1500/µL Platelets = ≥100 000/µL Hemoglobin = ≥9.0 g/dL or ≥5.6 mmol/La Renal Creatinine OR Measured or calculatedb creatinine clearance (GFR can also be used in place of creatinine or CrCl) = ≤1.5 × ULN OR ≥30 mL/min for participant with creatinine levels >1.5 × institutional ULN Hepatic Total bilirubin = ≤1.5 ×ULN OR direct bilirubin ≤ULN for participants with total bilirubin levels >1.5 × ULN AST (SGOT) and ALT (SGPT) = ≤2.5 × ULN (≤5 × ULN for participants with liver metastases) Coagulation International normalized ratio (INR) OR prothrombin time (PT) Activated partial thromboplastin time (aPTT) = ≤1.5 × ULN unless participant is receiving anticoagulant therapy as long as PT or aPTT is within therapeutic range of intended use of anticoagulants ALT (SGPT)=alanine aminotransferase (serum glutamic pyruvic transaminase); AST (SGOT)=aspartate aminotransferase (serum glutamic oxaloacetic transaminase); GFR=glomerular filtration rate; ULN=upper limit of normal. a Criteria must be met without erythropoietin dependency and without packed red blood cell (pRBC) transfusion within last 2 weeks. b Creatinine clearance (CrCl) should be calculated per institutional standard. Note: This table includes eligibility-defining laboratory value requirements for treatment; laboratory value requirements should be adapted according to local regulations and guidelines for the administration of specific chemotherapies.

Exclusion Criteria:

Participants are excluded from the study if any of the following criteria apply:

1. Stage 2, 3 or 4 Triple negative breast cancer

2. Hormone Receptor positive and/or Human Epidermal Growth factor 2 (HER 2) positivebreast cancer

3. A WOCBP who has a positive urine pregnancy test within 72 hours prior to dose ofstudy drug (see Appendix 2). If the urine test is positive or cannot be confirmed asnegative, a serum pregnancy test will be required.

4. Has received prior therapy with an anti-PD-1, anti-PD-L1, or anti-PD-L2 agent orwith an agent directed to another stimulatory or co-inhibitory T-cell receptor (eg,CTLA-4, OX-40, CD137).

5. Has received prior systemic anti-cancer therapy including investigational agentswithin 4 weeks to allocation of therapy.

6. Has received prior radiotherapy within 2 weeks of start of study intervention orradiation-related toxicities requiring corticosteroids. Note: Two weeks or fewer ofpalliative radiotherapy for non-CNS disease, with a 1-week washout, is permitted.

7. Has received a live vaccine or live-attenuated vaccine within 30 days before thefirst dose of study intervention. Administration of killed vaccines is allowed.

8. Has received an investigational agent or has used an investigational device within 4weeks prior to study intervention administration.

9. Has a diagnosis of immunodeficiency or is receiving chronic systemic steroid therapy (in dosing exceeding 10 mg daily of prednisone equivalent) or any other form ofimmunosuppressive therapy within 7 days prior to the first dose of study drug.

10. Known additional malignancy that is progressing or has required active treatmentwithin the past 3 years. Note: Participants with basal cell carcinoma of the skin,squamous cell carcinoma of the skin or carcinoma in situ, excluding carcinoma insitu of the bladder, that have undergone potentially curative therapy are notexcluded.

11. Has known active CNS metastases and/or carcinomatous meningitis.

12. Has severe hypersensitivity (≥Grade 3) to any of the components or any of itsexcipients used in the study treatments.

13. Has active autoimmune disease that has required systemic treatment in the past 2years except replacement therapy (eg., thyroxine, insulin, or physiologiccorticosteroid)

14. Has a history of (non-infectious) pneumonitis/interstitial lung disease thatrequired steroids or has current pneumonitis/interstitial lung disease.

15. Has an active infection requiring systemic therapy for >14 days from initiation ofC1D1 study treatment.

16. Concurrent active Hepatitis B (defined as HBsAg positive and/or detectable HBV DNA)and Hepatitis C virus (defined as anti-HCV Ab positive and detectable HCV RNA)infection. Note: Hepatitis B and C screening tests are not required unless:

• Known history of HBV and HCV infection

• As mandated by local health authority

17. Has not adequately recovered from major surgery or has ongoing surgicalcomplications.

18. Has a history or current evidence of any condition, therapy, or laboratoryabnormality or other circumstance that might confound the results of the study,interfere with the participant's participation for the full duration of the study,such that it is not in the best interest of the participant to participate, in theopinion of the treating investigator.

19. Has known psychiatric or substance abuse disorders that would interfere withcooperation with the requirements of the trial.

20. Is pregnant or breastfeeding or expecting to conceive or father children within theprojected duration of the study, starting with the screening visit through 120 daysafter the last dose of trial treatment.

21. Has had an allogenic tissue/solid organ transplant.

22. Participants with the following contraindications to doxorubicin therapy: recentmyocardial infarction; severe myocardial insufficiency; severe arrhythmias; previoustreatment with complete cumulative doses of doxorubicin, daunorubicin, idarubicin,and/or other anthracyclines and anthracenedione.