SPT for Sensitization of Collagen Dressing

Inclusion Criteria:

• Age: 18-70

• Sex: Male & Female

• Fitzpatrick: I-V, minimum 10% of panel Fitzpatrick V.

• Individuals who will be able to read, understand and give an informed consentrelating to the study they are participating in.

• Individuals who will be free of any dermatological or systemic disorder, which inthe Investigator's opinion, could interfere with the study results.

• Individuals who will be in general good health and who will complete a preliminarymedical history form mandated by the testing facility.

• Individuals who will be able to and agree to cooperate with the Investigator andclinical staff.

• Individuals who will agree to have test products applied in accordance with theprotocol and are able to complete the full course of the study.

• Individuals who have not participated in a similar study in the past 30 days.

• Individuals who agree to refrain from sun tanning/bathing and prolonged exposure tosunlight (outdoors).

• Individuals who are willing to refrain from wetting, wiping, cleanse, and coveringthe test sites (volar surface of the forearms) between the baseline and up to 48-hour time points.

• Female volunteers who are willing to undergo a urine pregnancy test.

• Individuals willing to refrain from vitamin C (Emergen-C), orange juice and vitaminwater for the study duration and two days prior to the SPT test.

Exclusion Criteria:

• Individuals who are currently taking any medications (topical or systemic) that maymask or interfere with the test results (specifically, corticosteroids, topicaland/or systemic [except nasal steroids], non steroidal anti-inflammatory drugs [e.g.ibuprofen, Advil, Motrin, aspirin > 325mg/day], antihistamines, and topical/oralimmunosuppressive medications).

• Individuals who have a history of any acute or chronic disease that might interferewith or increase the risk on study participation. (e.g., systemic lupuserythematosus, rheumatoid arthritis, HIV positive).

• Individuals who are diagnosed with chronic skin allergies (atopic dermatitis/eczema)or recently treated skin cancer within the last 12 months.

• Individuals who have damaged skin in close proximity to test sites (e.g., sunburn,uneven skin pigmentation, tattoos, scars, excessive hair, active acne papules orother disfigurations).

• Individuals who control their diabetes using insulin.

• Individuals with any history, which in the Investigator's opinion, indicates thepotential for harm to the subject or places the validity of the study in jeopardy.

• Female volunteers who indicate that they are pregnant or are planning to becomepregnant or nursing.

• Individuals with a known history of hypersensitivity to any cosmetics, personal careproducts, fragrances, and/or adhesives.

• Individuals that have a history of dermatographism.

• Individuals have a history of frequent skin irritation.

• Employees of ALS.

• Individuals with a known fish allergy.