Metagenomic Next-Generation Sequencing Guides Anti-Infection Strategies

Inclusion Criteria:

1. Confirmed HIV infection;
2. Progression to AIDS stage: CD4+ count <200 cells/ul and/or occurrence of AIDSopportunistic infections;
3. Age between 18 and 65 years;
4. Meeting the diagnosis of severe pneumonia or septic shock:
5. The criteria for severe pneumonia diagnosis refer to the 2021 ATS (AmericanThoracic Society) and IDSA (Infectious Diseases Society of America) guidelinesfor the treatment of community-acquired pneumonia;
6. The criteria for septic shock refer to the Chinese Society of Critical CareMedicine's "Chinese Guidelines for the Emergency Treatment of Sepsis/Septic Shock (2018).

Exclusion Criteria:

1. Coexisting central nervous system lesions, severe liver disease, or cirrhosis;
2. Concurrent AIDS-related or unrelated tumors;
3. Women who are pregnant or breastfeeding;
4. Severe underlying diseases of the heart, lungs, liver, kidneys, etc.;
5. Alcohol abuse or drug use;
6. The researcher believes that the overall condition of the subject affects theevaluation and completion of the trial.