Study on Early Auxiliary Diagnosis and Postoperative Recurrence Monitoring of Breast Cancer

The first stage Inclusion Criteria： Women between the ages of 18 and 80 who are not pregnant or lactating; Meet any of thefollowing categories:

1. Breast cancer Newly diagnosed breast cancer patients [according to breast cancer TNMstaging]; The patient was not accepted prior to blood collection Any breast cancerantitumor related treatment, including surgery, transplantation, radiation,chemotherapy, etc.
2. precancerous lesions Dysplasia, carcinoma in situ, atypical hyperplasia, etc., noprevious history of malignant tumor.
3. Benign lesions These include fibroadenoma of the breast, intraductal papilloma of thebreast, cystic hyperplasia of the breast, lobulated tumors of the breast, Plasma cellmastitis.
4. Healthy people No abnormal breast, no history of malignancy within 5 years; Age 50years or above.

Exclusion Criteria:

1. previous breast cancer;
2. A history of other cancers;
3. Patients who have received major surgical treatment such as blood transfusion ortransplantation within 3 months
4. Participate in other interventional clinical researchers, pregnant or lactating women,or patients suffering from autoimmune diseases, genetic diseases, mental disorders,etc., within 3 months.
5. Patients with other diseases deemed unsuitable for inclusion by the researcher; The second stage Inclusion Criteria：
6. Patients with definite pathological diagnosis of breast cancer who were to receiveradical surgery after preoperative evaluation for initial treatment
7. Women aged between 18 and 80 who are not pregnant or lactating;
8. The radical surgery to be received includes mastectomy radical surgery, simpleexcision + sentinel lymph node biopsy and modified radical surgery, and on this basis,mastectomy plastic surgery, stage I reconstruction and other operations that have thesame radical treatment effect and do not affect the entire standardized treatment ofpatients
9. Enrolled patients were newly treated breast cancer patients without prior malignanttumor history and treatment history Exclusion Criteria:
10. Stage IV breast cancer patients, or ECOG score > 2.
11. A history of malignant tumor or other hereditary diseases;
12. Have received organ transplantation, stem cell transplantation, bone marrowtransplantation or received blood transfusion within the past month;