Acute Exercise on Brain Insulin Sensitivity

Inclusion Criteria:

• Male or female >40 and <80 years old.

• Has a body mass index >25 and <45 kg/m2.

• Physical Activity (<150 min of moderate/high intensity exercise per week)

Exclusion Criteria:

• Subjects who have not been weight stable (>2 kg weight change in past 3 months)

• Subjects who are smokers or who have quit smoking <1 years ago

• Subjects with abnormal estimated glomerular filtration rate (eGFR).

• Hypertriglyceridemic (>400 mg/dl) and hypercholesterolemic (>260 mg/dl) subjects

• Hypertensive (>160/100 mmHg)

• Subjects with a history of significant metabolic, cardiac, congestive heart failure,cerebrovascular, hematological, pulmonary, gastrointestinal, liver, renal, orendocrine disease or cancer that in the investigator's opinion would interfere withor alter the outcome measures or impact subject safety.

• Pregnant (as evidenced by positive urine pregnancy test) or nursing women

• Subjects with contraindications to participation in an exercise

• Current Pregnancy

• Currently taking active weight suppression medication (e.g. phentermine, orlistat,lorcaserin, naltrexone-bupropion in combination, liraglutide, benzphetamine,diethylpropion, phendimetrazine)

• Subjects currently taking medications that affect heart rate and rhythm (i.e. Ca++channel blockers, nitrates, alpha- or beta-blockers).

• Known contraindications for MRI imaging