R-3750 in Patients with Mild to Moderate Ulcerative Colitis

INCLUSION CRITERIA:

• 18-65 years of age

• Ability to provide written informed consent

• Confirmed diagnosed with UC by colonoscopy and histology and suffering from mild tomoderate UC as defined by MMDAI with score of 3-9

• On a stable dose of aminosalicylate (i.e. no change in medication within 4 weeks ofstudy enrollment) and not planning to initiate new medication other than the studydrug

• For women of childbearing potential or men with a partner of childbearing potential,agree to use birth control methods (including hormonal contraceptives, intrauterinedevice (IUD) or hormone releasing system (IUS), vasectomy) and men will refrain fromdonating sperm during the study and at least 30 days after dosing (per FDAguidelines)

• For the expansion cohort, a flexible sigmoidoscopy is required, unless an endoscopywas completed within 3 months from enrollment is available

• Refrain from receiving any type of vaccinations during the study period (to includebut not limited to influenza, COVID, shingles, tetanus, hepatitis, pneumonia, HPV,DPT, MMR, and polio)

EXCLUSION CRITERIA:

• Pregnancy, planned pregnancy, breastfeeding women

• Evidence of severe UC disease (MMDAI score greater than or equal to 10)

• Evidence of any active or recent infection including chronic infectious disease suchas Hepatitis B, C, or HIV

• Evidence of any active or recent chronic chest infection with bronchiectasis orsinusitis, or covid-19 infection in the past 3 months

• Treatment with immunosuppressants or anti-cancer drugs, e.g., anti-TNF-α agents,anti-integrin agents, azathioprine or 6-MP, 6-thioguanine, methotrexate, ozanimod,tofacitinid, upadacitinib, tacrolimus, cyclophosphamide, or cyclosporine or anyother therapy that is not an aminosalicylate within the last 3 months

• Received an investigational drug within 3 months (or 5 half-lives, whichever islonger) before study entry

• Use of steroidal drugs to treat UC (e.g., prednisone >20 mg/day)

• Use of probiotics within the last one (1) week and during the trial.

• Treatment with systemic broad-spectrum antibiotics in the past 2 months

• Major active systemic autoimmune disease other than UC

• History of anaphylaxis or allergies to probiotics

• History of alcohol or drug abuse within the past 2 years

• History of stroke, or any cerebrovascular disease requiring medication/treatment

• History of cancer, apart from successfully treated basal cell carcinoma or in situcarcinoma of the cervix >1 year prior to enrollment

• Significant laboratory abnormalities, including liver transaminases (AST or ALT) > 1.5X the upper limit of normal.

• Second degree or higher heart block or clinically significant arrythmia

• Any other clinically significant renal, hepatic, hematological or other disease orlaboratory abnormality which, in the opinion of the investigator, would interferewith the conduct, the interpretation of the safety signals or results of the trial,or would place the subject at unacceptable risk

• Any condition or circumstance that, in the opinion of the Principal Investigator,would compromise the safety of the subject or the quality of study data