Individual Variability of Experimental Gingivitis Response

Inclusion Criteria:

• Age 18 to 35
• Caucasian
• No history of periodontitis/periodontal treatment (based on self-reported history)
• Medical history clear of cardiovascular disease, diabetes, cerebrovascular disease,kidney or liver disease, cancer, HIV, Tuberculosis and Hepatitis C (self-reported)
• Never smoker, e-cigarettes or nicotine gum use (self-reported). Individuals who rarelysocially smoked more than 5 years ago would be permitted to participate.
• Minimum of 24 teeth present
• Be willing and competent (verbally and cognitively) to give written informed consentand complete a medical history form
• Be willing and physically able to carry out all study procedures
• Not have had a scale and prophylaxis in the previous month or have one scheduled priorto the end of the study
• Participant in good periodontal health, based on:

1. absence of interproximal probing pocket depths (PPD) and clinical attachmentlevel (CAL) > 3mm (with the exception of distal surfaces of third molars in thepresence of impacted third molars) at the screening visit.
2. bleeding on probing (BOP) < 30% at the Screening visit and BOP <10% at Day -7.

Exclusion Criteria:

• Currently taking part in other clinical trials
• Pregnant or breastfeeding women
• Taking medications including systemic anti-inflammatory medication within 3 months ofthe study (NSAIDs, no more than once per week and not in the week before samplecollection, is permitted)
• Systemic antibiotic intake within 6 months
• Psychiatric conditions affecting participation in the study
• The participant is also a member of staff who is part of the study team
• Current orthodontic treatment
• Regular consumption of alcohol exceeding the government recommended levels
• Denture wearer/presence of dental implants/bridges
• Have oral piercings
• Obvious signs of untreated caries
• Taking dietary supplements (e.g. multivitamins; antioxidants; fish oils)