PBMC as Biomarkers of Diabetic Cardiomyopathy

Inclusion Criteria:

Inclusion criteria common to the 4 groups:

• Patient coming for a scheduled cardiology or endocrinology visit as part of theirfollow-up

• Patient coming on an empty stomach for the blood test

• Male or female aged between 40 and 80 inclusive

• Patient without systemic disease and/or without anti-inflammatory treatment

• Patient not objecting to this research

• Patient accepting the conservation of biological samples and the performance ofgenetic analyzes

Group 1: No-T2D +MS / No-HF (control group)

• Patient without T2D or MS and without heart failure coming to a consultation or dayhospital for another reason (e.g. screening for atypical symptom, etc.)

Group 2: No-T2D +MS / HFpEF or HFmrEF

• Patient without T2D or MS

• HFpEF or HFmrEF. diagnosed

Group 3: T2D+MS / no-HF

• Patient diagnosed with T2D+MS

Group 4: T2D +MS / HFpEF or HFmrEF

• Patient diagnosed with T2D and MS

• HFpEF or HFmrEF. diagnosed

Exclusion Criteria:

Non-inclusion criteria common to the 4 groups:

• History (ATCD) of cardiovascular disease (valvular (more than moderate valvedisease), radiation, post-cardiotoxic chemotherapy, amyloidosis, etc.) other thanHFpEF or HFmrEF

• Atrial fibrillation or permanent flutter ( paroxysmal AF is not a non-inclusioncriterion: the rhythm must be sinus at the time of the ultrasound)

• Systemic disease and/or anti-inflammatory treatment

• ATCD of known coronary artery disease

• Unbalanced hypertension (>160/100 mmHg)

• Pregnant and lactating women on questioning

• Persons deprived of their liberty by a judicial or administrative decision

• Persons subject to psychiatric care

• Patients subject to a legal protection measure (guardianship, curators)

• Subject participating in another interventional research including a period ofexclusion still in progress.

• Chronic kidney disease(GFR<30ml/min/1.73m2)

Group 1: No-T2D +MS / No-HF (control group)

• Presence of diabetes (whatever the type) and MS

• Presence of heart failure or other known heart disease

Group 2: No-T2D +MS / HFpEF or HFmrEF

• Presence of diabetes (whatever the type) and MS

• No Heart failure

• Left Ventricular Ejection Fraction (LVEF) on ultrasound ≤ 40%

Group 3: T2D+MS / no-HF

• Without diabetes or other type of diabetes than T2D

• Presence of Heart failure or other known coronary artery disease

Group 4: T2D +MS / HFpEF or HFmrEF

• Absence of diabetes or presence of another type of diabetes than T2D

• Absence of Heart failure

• LVEF on ultrasound ≤ 40%