DiAL Health Research Study

Inclusion Criteria:

1. Healthy male or non-pregnant pre-menopausal female, 25-45 years of age, inclusive

2. BMI ≥22kg/m2 and <30kg/m2

3. Screening lab values within normal limits, or deemed not clinically significant

4. Stable body weight (less than ± 2kg) in the past 6 months

5. Regularly wake up between 5-8 AM

Exclusion Criteria

1. History of prior bariatric surgery, active gallstone disease, extensive bowel resection or any disease or condition that seriously affects body weight and/or body composition

2. Regular (daily) use of medications for chronic medical conditions.

3. History or clinical manifestation of:

4. Significant chronic metabolic, hematologic, pulmonary, cardiovascular, gastrointestinal, neurologic, immune, hepatic, renal, urologic, circadian, sleep disorders, reproduction or other condition that would not be considered 'healthy' in opinion of the investigator

5. Anemia (Hb <10 g/dL or hematocrit < 30)

6. Diabetes or fasting glucose ≥ 126 mg/dL or HbA1c ≥ 6.5% at screening

7. Hypertension (Systolic Blood Pressure >140 mmHg & Diastolic Blood Pressure >90 mmHg)

8. Cancer requiring treatment in the past 5 years (except for non-melanoma skin cancer)

9. Low Bone mineral density z-score < -2.0 of the hip (total hip or femoral neck) or lumbar spine (L1-L4)

10. Depression as determined by the Beck Depression Inventory-II > 19

11. Pregnant, postpartum (< 12 months) or lactating females or attempting to become pregnant in the next 7 months

12. Current or history (within the past 5 years) of an eating disorder

13. Asthma or sleep apnea

14. Lifestyle related factors that would confound outcomes:

15. Non-conventional eating patterns (vegan, paleo, fasting, etc.) and eating duration (<11 hours per day)

16. Drug or alcohol abuse (up to 14 drinks a week are allowed) within the past 2 years

17. Current smoking, vaping or use of tobacco products within the past 6 months

18. Currently participating or recently engaged (4 consecutive weeks in the past 3 months) in heavy aerobic activity (e.g., jogging, running, or riding fast on a bicycle) ≥ 240 minutes per week or heavy resistance training ≥ 3 times per week that results in heavy breathing and sweating.

19. Perform overnight shift work > 1 day/week or plan to cross more than 2 time zones within 1 month of study assessments

20. Unwilling or unable to adhere to the rigors of the protocol or failure to complete the behavioral run-in task (at least 80% compliance)