Foot ulcer is a common complication in diabetic patients, in some cases requiring
hospitalization and may result in amputation. Effective treatment and care of diabetic foot
is as important as prevention. For this reason, the condition of the wound should be
evaluated well, effective wound care materials should be used, and current evidence-based
practices should be included in treatment and care. One of these current applications is PRF.
In the literature, the success and positive effects of PRF applications on dentistry, plastic
surgery, orthopedics, eye surgery, diabetic foot, chronic arterial and venous ulcers, and
complex wounds that are difficult to heal have been reported.
This study was planned as a prospective case-control study to examine the effect of PRF
application on wound healing in diabetic foot wound. The population of the study will consist
of patients with diabetic foot wounds, who applied to Bolu Home Health Services affiliated to
the Republic of Turkey Ministry of Health Bolu Provincial Health Directorate, and who were
followed up in the center of Bolu.
The sample of the study will consist of patients with diabetic foot wounds, who met the
inclusion criteria and agreed to participate in the study. It is planned to include 7
patients for the experimental group and 7 patients for the control group (14 patients in
total). Before starting the study, patients will be randomized in a computer program
(random.org). According to the randomization results of the program, the patients in the
first column will be the experimental group and the patients in the second column will be the
control group. If the patient is in the experimental group, PRF will be applied to the
diabetic foot wound, and in the control group, classic wound dressing (CWD) will be applied.
PRF Preparation and Application Technique (To be applied to the experimental group):
10 ml blood will be collected into a tube without anticoagulant with an injector or a
vacutainer directly, from the patients included in the experimental group. Then,
centrifugation will be carried out immediately at 3000 (rpm) speed for 10 minutes. This
process will be applied by the researcher next to the patient by using a Yuda 800D brand
desktop type centrifuge device with 6 tube capacity, 500-4000 rpm speed setting and 5-30
minutes time setting. After this procedure, the tube will be removed from the device and the
PRF clot formed in the middle of the tube will be removed with forceps. This clot will be
compressed into a membrane between two sterile gauzes moistened with saline, with as little
finger pressure as possible. The shaped PRF will be placed to cover the entire diabetic foot
wound under sterile conditions. Then the dressing will be closed by covering with sterile
gauze.These applications will be repeated weekly and applied for a total of 11 weeks.
Classic Wound Dressing Application:
Classic dressing will be applied to the diabetic foot wound of the patients in the control
group by the researcher. After the diabetic foot wound is evaluated, it will be irrigate with
saline on sterile conditions, cover with sterile gauze and fix with a plaster. This dressing
will be repeated weekly and applied for a total of 11 weeks.
Wound evaluation will be done at 1st (first day of application), 2nd, 4th, 8th and 12th
weeks. "Data collection form", "Wound observation and evaluation form", 'Diabetes
Self-Efficacy Scale', 'Diabetic Foot Care Self-Efficacy Scale', 'Medical Adherence Report
Scale' will be used in the data collection phase of the research. The evaluation of both feet
in terms of diabetic foot will be made using the 'Diabetic Foot Evaluation Form'. In
addition, diabetic foot care training will be given to all patients at the first meeting.
In the power analysis (G*Power 3.1.9.4) made considering the studies in the literature, it
was determined that at least 12 patients (6 experiment, 6 control) should be reached for the
study. Problems that may arise with the participants during the study were taken into
account, and it is planned to include at least 7 patients (14 in total) for each group, by
overestimating 1 person for each of the two groups. The data will be evaluated using a
statistical package program. Evaluation will be made by using various tests
(dependent/independent t-test, Mann Whitney U, Wilcoxon, Kruskal Wallis,
Dependent/Independent ANOVA, Friedman, etc.) and correlation tests, depending on whether the
data show a normal distribution with descriptive statistical methods. The significance level
to be used was determined as 0.05.