Investigation of Laser Assisted Drug Delivery of NanoDOX®

Inclusion Criteria:

• Subject must be able and willing to provide written informed consent and comply withthe requirements of the study protocol;

• Subject must be able to read and understand English;

• Any gender and any Fitzpatrick skin type;

• Age equal to or greater than 18 years old;

• Willing to sun protect treated area (sunscreen or cover the area with clothes) forthe duration of enrollment in the study and 1 year after treatment.

• Subjects must be willing to undergo skin biopsies

Exclusion Criteria:

• Participation in another investigational drug or device clinical trial in the past 30 days;

• Are pregnant or lactating;

• History of allergic reaction to topical or local anesthesia;

• History of allergic reaction to Doxycycline or other tetracyclines;

• Regular intake of high doses of anti-inflammatory drugs (aspirin >81 mg/day,ibuprofen, corticosteroids, etc.) , immunosuppressive drugs or biologics;

• Clinically significant abnormal findings or conditions which might, in the opinionof the Investigator, interfere with study evaluations or pose a risk to subjectsafety during the study;

• Laser treatment in past six months; History of poor wound healing;

• History of keloids; History of extreme photosensitivity;

• History of severe hyperpigmentation; History of tattoos on thighs.