Pathogenesis of Kidney Disease in Type 1 Diabetes: a Modern Kidney Biopsy Cohort (The PANDA Study)

Phase

N/A

Condition

Diabetes Mellitus, Type 1

Diabetes Prevention

Diabetic Kidney Disease

Treatment

Iohexol Inj 300 MG/ML Diagnostic aid/agent used to measure glomerular filtration rate (GFR)

Aminohippurate Sodium Inj 20% Diagnostic aid/agent used to measure effective renal plasma flow (ERPF)

Clinical Study ID

NCT05319990

22-0250

Ages > 18
All Genders

Study Summary

Diabetic kidney disease (DKD) occurs in up to 40% of people with type 1 diabetes (T1D), often leading to kidney failure and markedly magnifying risks of cardiovascular disease and premature death. Landmark T1D kidney biopsy studies identified the classic pathological lesions of DKD, which have been attributed largely to hyperglycemia. Recent advances in continuous glucose monitoring (CGM) and automated insulin delivery have facilitated improved glycemic control, but the residual risk of DKD continues to be high. In addition, obesity and insulin resistance (IR) have accompanied intensive glycemic therapy and may promote mitochondrial dysfunction and inflammation. Deciphering the molecular underpinnings of DKD in modern-day T1D and identifying modifiable risk factors could lead to more effective and targeted therapies to prevent DKD.

Eligibility Criteria

Inclusion

Inclusion Criteria:

Age ≥ 18 years at enrollment (rationale: this study focuses on determinants of earlyDKD over the course of T1D in adults)
T1D duration >5 years (rationale: DKD in T1D rarely manifests prior to 5 years ofdisease duration)
HbA1c <11% (rationale: HbA1c ≥ 11% exceeds the average HbA1c at most academic centerand would limiting the generalizability of our study findings)

Exclusion

Exclusion Criteria:

T2D and monogenic diabetes (rationale: our study focuses on T1D)
Recent diabetic ketoacidosis, i.e., <1 month (rationale: safety and insulinresistance and tubular dysfunction of DKA can confound study findings)
eGFR < 30 ml/min/1.73m2 or dialysis treatment (rationale: to reduce the likelihoodof identifying secondary pathways that are not specific to kidney injury from T1D)
Kidney transplant recipients (rationale: molecular confounding fromimmunosuppression)
Kidney biopsy contraindications (rationale: safety - kidney biopsy):
Evidence of bleeding disorder or complications from bleeding
Use of aspirin, Nonsteroidal anti-inflammatory drugs (NSAIDS) or other bloodthinner that cannot be safely stopped for a sufficient time before and afterthe biopsy to avoid additional risk of bleeding.
INR > 1.4
Hemoglobin (Hgb) < 10 mg/dL (Colorado) [altitude]
Hemoglobin (Hgb) < 9 mg/dL (Washington)
Platelet count < 100,000 / µL
Uncontrolled or difficult to control hypertension (> 150/90 mmHg at the day ofbiopsy)
Single kidney (either by history, documented by prior imaging or ultrasoundperformed prior to the biopsy)
Kidney size: One or both kidneys < 8 cm
Hydronephrosis or other important renal ultrasound findings such as significantstone disease
Any evidence of a current urinary tract infection as indicated on day of biopsy
Clinical evidence of non-diabetic renal disease
Positive urine pregnancy test or pregnancy

Study Design

Iohexol Inj 300 MG/ML Diagnostic aid/agent used to measure glomerular filtration rate (GFR)

March 09, 2022

December 31, 2027

Study Description

The overall goal of this project is to characterize the molecular, morphometric, and metabolic features of DKD over the modern clinical course of T1D. The investigators hypothesize that perturbed kidney energetics and hypoxia are central metabolic pathways in the development of DKD. Kidney hypoxia stems from a mismatch between increased renal energy demand (e.g., increased glomerular filtration rate [GFR] and tubular reabsorption of sodium) and impaired substrate metabolism (e.g., IR and mitochondrial dysfunction) and results in activation of the hypoxia-inducible factor (HIF) system. Although upregulation of HIF1α confers favorable short-term effects, sustained activation promotes cellular injury. Supporting these hypotheses, data from our group and others have shown that obesity, IR, and The investigators also found that youth with T1D exhibit kidney hypoxia by MRI that strongly associates with IR and mitochondrial dysfunction. Moreover, our preliminary single-cell RNA sequencing (scRNA-seq) data from kidney biopsies demonstrate upregulated tubular expression of HIF1α in adults with T1D vs. healthy controls. The investigators propose to build a unique new longitudinal kidney biopsy cohort (N=100) spanning the critical duration of T1D over which DKD initiates and progresses (5-30 years). Participants will be enrolled from our existing CROCODILE study (adding longitudinal follow-up to completed kidney biopsies and baseline data and biosample acquisition) and from new enrollment at the University of Colorado and University of Washington. Normative kidney biopsy data will be provided from our existing cohort of healthy controls (N=20) and the Kidney Precision Medicine Project (KPMP). The investigators will implement state-of-the-art molecular (scRNA-seq) and morphometric interrogation of kidney tissue and rigorous metabolic phenotyping to include kidney magnetic resonance imaging (MRI), direct measurements of glycemia (continuous glucose monitoring), intraabdominal fat (dual-energy X-ray absorptiometry), estimation of insulin sensitivity by a T1D-validated equation, and (in a subset) GFR (iohexol clearance) and renal plasma flow (p-aminohippurate clearance). Participants will be followed longitudinally for DKD outcomes. A subset of 20 participants will undergo repeat kidney biopsies and associated procedures 3 years after baseline kidney biopsy.

Connect with a study center

Children's Hospital Colorado
Aurora, Colorado 80045
United States
Site Not Available

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