Evaluation of Kisspeptin Glucose-Stimulated Insulin Secretion With Oral Glucose Tolerance Test

Inclusion Criteria:

History:

• over the age of 18,

• normal pubertal development

• stable weight for previous three months,

• normal body mass index (BMI between 18.5-25)

• regular menstrual cycles

Physical examination:

• systolic BP < 140 mm Hg, diastolic < 90 mm Hg

Laboratory studies: (per Massachusetts General Hospital reference ranges)

• normal hemoglobin

• hemoglobin A1C < 6.5%

• blood urea nitrogen, creatinine not elevated

• aspartate aminotransferase, alanine aminotransferase < 3x upper limit of normal

Exclusion Criteria:

• active illicit drug use,

• history of a medication reaction requiring emergency medical care,

• difficulty with blood draws.

• history of chronic disease, except well controlled thyroid disease,

• recent use of prescription medications which interfere with metabolism orreproduction (recent = within 5 half-lives of the drug). Use of levothyroxine orseasonal allergy medications is acceptable,

• history of diabetes in a first degree relative,

• use of contraceptive pills, patches or vaginal rings within last 4 weeks.

• hyperlipidemia by fasting lipid panel

• positive serum pregnancy test (for all women)