A Clinical Trial Evaluating the Safety and Efficacy of a Single Subretinal Injection of AGTC-501 in Participants With XLRP

General Inclusion Criteria:

1. Provide written informed consent or assent (per local regulation), prior to theconduct of any study-related procedure. Participants who provide assent must have aparent, guardian, or legal representative provide written informed consent.

2. Be between 12 and 50 years of age (inclusive) at the time of informed consent andassent (as applicable).

3. Be male (XY chromosome) and have at least one documented pathogenic or likelypathogenic variant in the RPGR gene.

4. Have a clinical diagnosis of XLRP.

5. Be able and willing, as assessed by the Investigator, to follow study instructions,complete study assessments, comply with the protocol, and attend study visits forthe duration of the study. Ocular Inclusion Criteria (Study Eye):

6. Have a BCVA ≤ 78 letters (approximately Snellen, 20/32) and ≥ 34 letters (approximately Snellen, 20/200)

7. Have a LLVA ≤64 letters (approximately Snellen 20/50) in the study eye

8. Be able to perform all tests of visual and retinal function and structure in botheyes based on the participant's reliability, and fixation, in the study eye per theInvestigator's discretion.

9. Have an LLD of > 10 letters in the study eye

10. Have detectable baseline mean macular sensitivity measured by MAIA microperimetry,between 1-12 decibels (dB) in the study eye, as determined by the Investigator andconfirmed by the CRC with fixation loss ≤20% at each screening visit.

11. Have a detectable sub-foveal EZ line in the study eye as assessed by spectraldomain-optical coherence tomography (SD-OCT) and confirmed by the CRC.

General Exclusion Criteria:

1. Have other known disease-causing mutations documented in the participant's medicalhistory or identified through a retinal dystrophy gene panel, that in the opinion ofthe Investigator would interfere with the potential therapeutic effect of the studyagent or the quality of the assessments.

2. For participants with herpes simplex virus (HSV):

3. Have history of oral or genital herpes and unable and/or unwilling to utilizeprophylactic antiviral medication.

4. Have a history of ocular herpes.

5. Have active oral or genital herpes or are currently receiving treatment for HSVinfection.

6. Have known sensitivity or allergy to systemic corticosteroids or otherimmunosuppressive medications.

7. Have used anti-coagulant agents that may alter coagulation

8. Have used systemic corticosteroids or other immunosuppressive medications within 3months prior to screening and/or intend to use during screening. Corticosteroidsused on an as-needed basis administered by insufflation, inhalation or localadministration to the skin

9. If sexually active or planning to become sexually active, are unwilling to usebarrier contraception for 3 months following treatment administration.

10. Are currently participating or recently participated in any other research

11. Have previously received any AAV gene therapy product, stem cell therapy, cell-basedtherapy, or similar biologics.

12. Have significant media opacity impacting evaluation of the retina or vitreous.administration.

13. Had intraocular surgery within 90 days of study treatment administration.

14. Have any active ocular/intraocular infection or inflammation

15. Have a history of corticosteroid-induced raised IOP of >25 mmHg followingcorticosteroid exposure, despite topical IOP-lowering pharmacologic therapy.

16. Have any artificial retinal implant or prosthesis.

17. Have absence of clear ocular media and/or inadequate pupil dilation to facilitategood quality SD-OCT images.

18. Have any history of rhegmatogenous retinal detachment.

19. Have myopia (spherical equivalent) exceeding -10 diopters (or axial length of >30 mmif the Principal Investigator [PI] deems it appropriate to measure) or presence ofpathologic myopia in the study eye.

20. Have passed the Low Contrast Ora-VNC mobility course at ≤0.35 lux light level ineither eye or binocularly at any screening visit.