The Culture of Ovarian Cancer Organoids and Drug Screening

Inclusion Criteria:

1. Ovarian cancer patients who will accept primary cytoreductive surgery, withhistopathological type: low/high grade serous carcinoma, clear cell carcinoma,endometrioid carcinoma, mucinous carcinoma.
2. ECOG score 01,age 1870 years old
3. Expected survival over 6 months
4. The serum or urine pregnancy test must be negative within 7 days before enrollment forthe women of childbearing age who should agree that contraception must be used duringthe trial
5. CBC：Hb≥70g/L、WBC≥3.5×109/ L 、ANC≥1.5×109/L、PLT≥80×109/L；
6. Serum ALT≤2×UL, AST≤2×ULN；Serum creatinine≤1.5×ULN；

Exclusion Criteria:

1. Activity or uncontrol severe infection
2. Liver cirrhosis, Decompensated liver disease
3. History of immune deficiency, including HIV positive or suffering from congenitalimmunodeficiency disease
4. Chronic renal insufficiency or renal failure
5. Has combined with other malignant tumor which diagnosed within 5 years and/or neededto be treated
6. Myocardial infarction, severe arrhythmia and NYHA (New York heart association)≥2 forcongestive heart failure
7. During the treatment for complications, the drugs which lead to serious liver and/orkidney function impairment need to be used, such as tuberculosis