Characterizing Matrix Metalloproteinase-12 (MMP12) in Sputum

Inclusion Criteria (COPD):

• ≥40 years of age

• Current or ex-smokers with a >10 pack year smoking history

• Have a post-bronchodilator forced expired volume in 1 second (FEV1)/forced expiredvital capacity (FVC) ratio of <70% and a post-bronchodilator FEV1 value from ≥30%predicted (GOLD 1, 2 and 3), (Global Initiative for Obstructive Lung disease)

• Have a radiologist confirmed pulmonary emphysema diagnosis based on CT

Inclusion criteria for normal controls:

• No clinically significant medical condition or a history of asthma, COPD, cysticfibrosis, or other significant respiratory disorder including significantoccupational or environmental exposures with ongoing respiratory symptoms.

• No current or past smoking history

• Have a post-bronchodilator FEV1/FVC ratio of >70%

Exclusion Criteria:

Any potential subject who meets any of the following criteria will be excluded from participating in the study:

• Patients with other non-COPD airway diseases

• Patients with very severe COPD (FEV1<30% predicted)

• Patients with an intercurrent exacerbation

• Patients with life expectancy less than 3 months

• Pregnant or breastfeeding

• Undergoing immunomodulatory or biologic treatment

• Use of systemic steroids in the last month

• Hospitalization in the last 12 months due to exacerbation

• Known cardiovascular comorbidity under treatment or with hospitalizations of thiscause in the last year

• That they cannot perform spirometry

• Active malignancy

• Realization of lung surgery during the study period

• History of alcohol and drug abuse that prevents compliance with follow-up

• History of bronchial thermoplasty

• Participating in another study concomitantly

• MRI Related: patients who have implanted mechanically, electrically or magneticallyactivated device or any metal in their body which cannot be removed, including butnot limited to pacemakers, artificial limb, metallic fragments of foreign body,shunt, surgical staples (including clips or metallic sutures and/or ear implants).