Single Patient Classifier (SPC) Prediction for Stage II and III Advanced Gastric Cancer

Inclusion Criteria:

1. Sample providers criteria

• Male and female adult patients aged 19 years or over

• Postoperative patients with gastric cancer invading the submucosal layer andhaving 3 or more lymph nodes metastasis or who with gastric cancer infiltrationof muscularis propria and lymph node metastasis (stage II and III advancedgastric cancer patients, 8th of the AJCC)

• Patients who have not received neoadjuvant chemotherapy and radiotherapy

• Patients with pathological record and clinical information after surgery fromarchived FFPE(formalin-fixed paraffin-embedded) samples between 2005 and 2010

• Patients who have not been included in discovery clinical trial andconfirmatory clinical trial

• Patients who have undergone a radical gastrectomy and who show no evidence ofresidual tumors as observed with the unaided eye or through a microscope

2. Sample criteria

• The FFPE tumor specimens in storage have a tumor amount of at least 20% andtherefore can be tested.

• The quantity (not less than 400ng) and quality (A260/280 of not less than 1.8)of RNA are sufficient for analysis.

Exclusion Criteria:

1. Sample providers criteria

• Male and female patients aged less than 19 years

• Postoperative patients with gastric cancer invading the mucosa and submucosallayer and having less than 3 lymph nodes metastasis or who with gastric cancerinfiltration of muscularis propria without lymph node metastasis (stage II andIII advanced gastric cancer patients, 8th of the AJCC)

• Patients who have received neoadjuvant chemotherapy or radiotherapy

• Patients without pathological record and clinical information after surgeryfrom archived FFPE samples between 2005 and 2010

• Patients who have been included in discovery clinical trial and confirmatoryclinical trial

• Patients with residual tumors after surgery

2. Sample criteria

• The FFPE tumor specimens in storage have a tumor amount of less than 20% andtherefore cannot be tested

• The quantity and quality of RNA are not sufficient for analysis