The assessment of the safety and tolerability of the combined oral thiamine and biotin
therapy in patients with HD will be performed by:
Periodic clinical examination and anamnesis directed by a neurologist during
face-to-face and non-contact visits.
The collection of adverse effects during interviews of face-to-face visits to assess
tolerability.
Analytical monitoring with hematological and biochemical control (hepatic and renal
function) during the dose escalation period, subsequently being this periodic
control every 3 months, from signature of informed consent until the end the follow
up.
The evaluation of the biological efficacy of treatment with combined oral thiamine and
biotin therapy in increasing thiamine monophosphate (TMP) levels in cerebrospinal fluid
(CSF) of patients with HD is to be performed by:
The determination of thiamine levels: free thiamine, TMP, and thiamine pyrophosphate
(TTP) in CSF and blood of patients at the beginning and after the end of the study.
Comparison of thiamine levels (free, TMP and TTP) between the start and the end of
the study in CSF and serum of patients with HD.
The evaluation of the biological efficacy of the treatment with combined oral thiamine
and oral biotin therapy in neurodegeneration produced in HD will be performed by:
Measurement of neurofilament light chain protein (NfL) levels in CSF at the baseline
visit and after the end of the treatment.
The obtained score in the motor and Total Functional Capacity (TFC) section of the
Unified Huntington's Disease Rating Scale (UHDRS).
Measurement of bradykinesia through quantitative motion measurement techniques
(Quantitative motor assessment, Q-motor). Q-motor assessment relies on
three-dimensional position sensors and pre-calibrated force translators, for
standardized movement recording. This measurement will be made to patients in the
pre-selection visit, randomization, and quarterly face-to-face visits.
Score on the quality of life scale (SF-36).
Variation in the overall clinical impression scale of the patient and the examiner.
Measurement of the change in the volume of the caudate nucleus, white matter, and
cortical thickness, as well as in the change of the Combined cerebral atrophy score.
Measurements of cerebral structures will be acquired on magnetic resonance imaging
scans (3T) at the baseline visit and after the end of the treatment.
To determine the sample size required to examine secondary and exploratory objectives, we
based our estimations on the published thiamine-biotin treatment effects in preclinical
HD models, and on the reported differences in CSF thiamine levels between HD patients and
healthy subjects (Pico S, et al. 2021). According to the previous results, it is expected
that a medium-to-high effect size (0.6 ≤ Cohen's d ≥ 0.8) would be necessary to restore
TPP, TMP and Free-thiamine levels in CSF after treatment. Based on the parameter choices,
for a desired power of 0.80 and a Type I error rate of 0.05, we estimate that we would
need 24 HD patients to detect a standardized mean difference of 0.6. Sample size analysis
was conducted using GPower 3.1.9.7 software.
The demographic data collection as well as the information related to all the variables
analyzed during the study will be done through an electronic data collection notebook.
The notification of adverse effects, severity, and relationship with study medication
will be done through an electronic data collection notebook.
All statistical analyses will be conducted using SPSS v.26.0. IBM software and R studio
software package. Linear regression will be used when the variables are quantitative (eg,
scale measurements, CSF thiamine or NfL levels, among others) controlling for age, sex,
CAG repetitions and motor symptom severity (UHDRS and UHDRS-Total Functional Capacity) at
baseline as potential confounding variables. Logistic or multinomial regression when the
variables are groups (binary or multinomial).
We will examine the association between the severity of disease and CSF thiamine among HD
patients by fitting a linear mixed model for each clinical measurement, with age, sex,
CAG repetitions and disease severity (UHDRS-TFC) as the covariates.
Magnetic resonance imaging performed during the study will be processed with specific
neuroimaging programs for volumetry, diffusion and cortical thickness.