ATHN Transcends: A Natural History Study of Non-Neoplastic Hematologic Disorders

Participants who meet the following inclusion criteria and none of the exclusion criteria are eligible for enrollment in the base study:

Inclusion Criteria:

1. Any age

2. Having a congenital or acquired non-neoplastic hematologic disorder; or

3. Having a bleeding phenotype as indicated by an age adjusted abnormal ISTH BleedingAssessment Tool score with an unknown diagnosis; or

4. Connective tissue disorder with bleeding tendency as indicated by an age adjustedabnormal ISTH Bleeding Assessment Tool score.

Exclusion Criteria:

1. Does not qualify for inclusion in a cohort 2. Unable to give informed consent orassent 3. Unwilling to perform study procedures

Cohort Participant Selection

Each participant is to be enrolled in the cohort for which they qualify as defined below.

Hemophilia Cohort

Inclusion Criteria:

Participants who meet any of the following inclusion criteria are eligible for enrollment into this cohort:

1. Factor VIII or factor IX activity < 50%, without another explanation for lowclotting factor other than congenital hemophilia or being a known carrier forcongenital hemophilia; OR

2. Being a known carrier for congenital hemophilia with a factor VIII or factor IXactivity greater than or equal to 50% with or without a bleeding phenotype asindicated by an age-adjusted abnormal ISTH Bleeding Assessment Tool score; OR

3. Known congenital hemophilia that have a factor level >50% after receiving vector; OR

4. Acquired hemophilia

Exclusion Criteria:

None

Von Willebrand Disease Cohort

Inclusion Criteria:

Participants who meet the following inclusion criteria are eligible for enrollment into this cohort:

1. Meeting the definition of VWD or low VWF per most recent international guidelines

Exclusion Criteria:

None

Congenital Platelet Disorders Cohort

Inclusion Criteria:

Participants who meet the following inclusion criteria are eligible for enrollment into this cohort:

1. Abnormalities of platelet function

2. Glanzmann thrombasthenia (GPIIb or GPIIIa)

3. Bernard-Soulier syndrome (GPIbalpha, GPIbbeta, or GPIX)

4. Abnormalities of platelet granules

5. Abnormalities of platelet signal transduction

6. Abnormalities of platelet secretion

7. Collagen Receptor Defect

8. ADP Receptor Defect

9. Thromboxane Receptor Defect

10. Giant Platelet Disorder

11. Abnormalities in platelet aggregation testing due to another or unknown cause (notdrug related)

Exclusion Criteria:

Platelet disorders secondary to medications or other substances

Rare Disorders Cohort

Inclusion Criteria:

Participants who meet the following inclusion criteria are eligible for enrollment into this cohort:

1. Have an established Rare Coagulation Disorder (RCD) diagnosis of one of thefollowing:

2. PAI-1 deficiency

3. Factor I, II, V, VII, X, XI, XIII deficiencies

4. Combined FV and FVIII deficiency

5. Plasminogen deficiency

6. Decreased tissue plasminogen activator

7. Afibrinogenemia/hypofibrinogenemia/dysfibrinogenemia

Exclusion Criteria:

None

Bleeding NOS Cohort

Inclusion Criteria:

Participants who meet the following inclusion criteria are eligible for enrollment into this cohort:

1. Have a bleeding phenotype as indicated by an age-adjusted abnormal ISTH BleedingAssessment Tool score with an unknown diagnosis; OR

2. Connective tissue disorder with bleeding tendency as indicated by an age-adjustedabnormal ISTH Bleeding Assessment Tool score

Exclusion Criteria:

None

Thrombosis/Thrombophilia Cohort

Inclusion Criteria

Participants who meet the following inclusion criteria are eligible for enrollment into this cohort:

1. Have a prior history of arterial or venous thrombosis

2. Patients with a known congenital or acquired thrombophilia with or without athrombosis a. Common congenital thrombophilias:: i. Protein C deficiency ii. ProteinS deficiency iii. Antithrombin deficiency iv. Factor V Leiden v. Prothrombin genemutation b. Rare genetic factors i. Hyperhomocysteinemia c. Indeterminate geneticfactors i. Elevated factor VIII ii. Elevated factor IX iii. Elevated factor XI iv.Elevated lipoprotein (a) d. Acquired thrombophilias i. Lupus anticoagulant ii.Anti-cardiolipin antibodies/Beta2 glycoprotein antibodies iii. Antiphospholipidsyndrome

Exclusion Criteria

1. Acquired thrombophilia secondary to medications (birth control pills or hormonereplacement therapy, overweight or obesity, smoking, cancer, pregnancy, surgery,injury, prolonged inactivity/bedrest, heart failure, inflammatory bowel disease, orkidney disease

Non-Neoplastic Hematologic Conditions Cohort

Inclusion Criteria

Participants who meet the following inclusion criteria are eligible for enrollment into this cohort:

1. Having any congenital or acquired non-neoplastic hematologic disorder not includedin any other cohort

Exclusion Criteria

None

Previously Untreated Patients Arm Eligibility Criteria

Inclusion Criteria Diagnosis of congenital hemophilia A (FVIII <40%) or hemophilia B (FIX <40% or below lower limit for age)

1. Age <18 years at time of enrollment

2. Parent or authorized guardian or legally authorized representative (LAR) can provideinformed consent

3. Care established at one of the participating HTCs

Exclusion Criteria

1. CFC exposure, fresh frozen plasma (FFP), cryoprecipitate, and single donor platelets >3 EDs

2. Concomitant diagnosis with another bleeding disorder

3. History of confirmed inhibitor

INHIBIT Module Eligibility Criteria:

Inclusion Criteria

1. Diagnosis of severe factor VIII deficiency with baseline factor VIII level <1%

2. Initiating or plan to initiate prophylaxis with emicizumab or factor replacement

3. Factor exposure, plasma/FFP, cryo, and single donor platelets ≤3 EDs

4. ≤5 years of age

Exclusion Criteria

1. Concomitant diagnosis with bleeding disorder other than hemophilia A

2. Immune disorder

3. Factor exposure, plasma/FFP, cryo, and single donor platelets >3 EDs

4. Previous history or presence of factor VIII inhibitor

ALTUVIIIO® Module Eligibility Criteria:

Inclusion criteria:

• People with severe HA with a baseline FVIII activity of less than 1%. (Whileinclusion for participation in ATHN Transcends lists <5% FVIII activity, thisproposed module will limit enrollment to people with FVIII activity levels of <1%)

• <18 years of age

• No history of FVIII inhibitor

• Sex at birth of male, female, or intersex

• Participants may be exposed to unfractionated blood components, no more than onedose of FVIII concentrate other than efanesoctocog alfa and up to three doses ofefanesoctocog alfa prior to enrollment

• Potential participants who have a history of bleeding will be eligible forparticipation if they meet all other inclusion criteria

Exclusion criteria:

• Not meeting all the inclusion criteria

• Any exposure to blood components or FVIII replacement products except as describedin the inclusion criteria

• History of positive inhibitor testing

• History of hypersensitivity reactions associated with efanesoctocog alfaadministration

• Any concurrent clinically significant major disease that, in the investigator'sopinion, would make the participant unsuitable for enrollment.

• The presence of any additional inherited bleeding disorder diagnosis

• Enrollment in a concurrent clinical interventional drug study

• Intake of an Investigational Medicinal Product within three months prior toinclusion in this study

• Inability to comply with study requirements

• Other, unspecified reasons that, in the investigator's opinion, make the participantunsuitable for enrollment.

Hemophilia Natural History Arm Eligibility Criteria:

Inclusion Criteria

1. Congenital hemophilia A or B of any severity with or without inhibitors receiving acurrent therapy, a non-factor product, or for whom use of a non-factor product is apossibility.

Exclusion Criteria

1. Presence of any known bleeding disorder other than congenital hemophilia A or B

2. Presence of concurrent hemophilia and a second hemostatic defect (low Von WillebrandFactor (VWF) without VWD diagnosis is not excluded)

3. Unable or unwilling to comply with the study arm protocol.

Rebinyn® Module Eligibility Criteria

Inclusion Criteria:

Participants who meet the following inclusion criteria at the time of screening are eligible to enroll in the study module.

Rebinyn® Cohort

1. Has provided signed written consent for the Rebinyn® Module before any study-relatedactivities.

2. Male participants, at any age with hemophilia B, naïve or minimally exposed (up to 3EDs) to nonacog beta pegol treatment at time of study enrollment.

3. Decision to initiate treatment with commercially available nonacog beta pegol hasbeen made by the participant(s)/Legally Authorized Representative(s) (LAR(s)) andthe treating physician before and independently from the decision to include theparticipant in this study.

Rebinyn® RWE Cohort

1. Has provided signed written consent for ATHN Transcends Study before anystudy-related activities.

2. Currently treated with nonacog beta pegol

3. Any age

Exclusion Criteria:

Participants who fall into any of the following exclusion criteria at the time of screening are not eligible for enrollment into the study module:

Rebinyn® Cohort

1. Previous participation in this study. Participation is defined as having giveninformed consent in this study.

2. Mental incapacity, unwillingness or language barriers precluding adequateunderstanding or cooperation, including a diagnosis or suspicion of attentiondeficit hyperactivity disorder (ADHD) or autism spectrum disorder (ASD) per thediscretion of the Principal Investigator.

3. Known or suspected hypersensitivity to nonacog beta pegol or related products.

4. Clinical suspicion or presence of FIX inhibitor at time of inclusion.

5. Inability or unwillingness to undergo the neurological assessment/ structureddevelopmental history.

Rebinyn® RWE Cohort 1. None

Hemophilia Gene Therapy Outcomes Arm Eligibility Criteria:

Inclusion Criteria

1. Hemophilia A or B of any severity with or without inhibitors having received or willreceive a hemophilia gene transfer product in the next 6 months.

2. Age 18 years and older

3. Able to give informed consent. Exclusion Criteria None HEMGENIX® Module Eligibility Criteria Inclusion Criteria: HEMGENIX Cohort • Age 18 years of age or older

• Treatment with commercial HEMGENIX

• Have provided signed written informed consent within 3 months before or within 6months after HEMGENIX treatment, or within 6 months of when the study is initiatedat the treating site. Exclusion Criteria, both cohorts:

• Have been treated with etranacogene dezaparvovec in a clinical trial. Congenital Platelet Disorders Arm Eligibility Criteria: Inclusion Criteria

1. Platelet adhesion defect

2. Bernard Soulier syndrome (Defective GPIb-IX-V receptor, impaired adhesion toVWF)

3. Velocardio-facial syndrome/DiGeorge syndrome (Defective GPIb-IX-V receptor)

4. Platelet type vWD (Defective GPIb-IX-V, gain of function interaction betweenVWF-GP1bα)

5. Platelet aggregation defect

6. Glanzmann thrombasthenia (Defective integrin αIIbβ3 (GPIIb/IIIa)

7. Platelet aggregation defect, NOS

8. Agonist receptor defects

9. Epinephrine

10. ADP

11. Collagen

12. Thromboxane A2

13. Platelet signaling defects

14. Cyclooxygenase deficiency (PTGS1 mutation)

15. Phospholipase A2 deficiency

16. Thromboxane synthase deficiency (TBXAS1 mutation)

17. G protein activation defect (GNAS mutation)

18. Scott syndrome (defect in phosphatidyl serine translocation)

19. Platelet Granule disorders

20. Dense granule storage pool disorder • Hermansky Pudlak syndrome

• Chediak Higashi syndrome
• Griscelli syndrome

2. Alpha granule storage pool disorder • Grey platelet syndrome

• Arthrogryposis-Renal Dysfunction-Cholestasis (ARC) syndrome
• Quebec platelet disorder
• Paris-Trousseau syndrome

3. Combined alpha delta granule deficiency

4. Platelet cytoskeletal structure defects

5. Wiskott Aldrich syndrome

6. MYH9 associated disorders (myosin heavy chain) • May Hegglin syndrome • Fechtner syndrome

• Sebastian syndrome
• Epstein syndrome

3. Other mutations • FLNA mutations (Filamin) • DIAPH1 (Actin and microtubules) •ACTN1 (alpha actinin) • TPM4 (tropomyosin)

• TUBB1 (beta tubulin)

7. Other Congenital thrombocytopenias

8. Familial platelet disorders and predisposition to AML (RUNX1)

9. X linked thrombocytopenia with dyserythropoiesis (GATA1)

10. Congenital amegakaryocytic thrombocytopenia (MPL)

Exclusion Criteria

1. Diagnosis of von Willebrand disease (Meeting the definition of VWD or low VWF permost recent international guidelines)

2. Diagnosis of Hemophilia A or Hemophilia B (Factor VIII or IX ≤ 40%) FIX Prophylaxis Cohort

• Age 18 years of age or older

• Treatment with FIX prophylaxis therapy

• Has provided signed written consent at any time for ATHN Transcends Study Glanzmann Thrombasthenia (GT) Module Eligibility Criteria: Inclusion Criteria

1. Participant has signed the informed consent/assent form 2. Participant has flowcytometry or aggregometry or genetics confirmed GT 3. Participant is willing toperform study procedures, including daily bleed tracking for 3 months

Exclusion Criteria None