China Alzheimer's and Neurodegenerative Disorder Research

1. The inclusion and exclusion criteria of AD group. 1.1 The AD group inclusion criteria: 1.1.1 Aged 40-100 years old (≥ 40 years old, ≤ 100 years old). 1.1.2 Diagnosed with AD according to Alzheimer disease diagnosticcriteria following NINCDS-ADRDA1984 or NIA-AA 2011 guideline. 1.1.3 Had adequate hearing, vision and comprehension and verbal expression to completethe cognitive assessments. 1.1.4 Had at least 3 years of education. 1.1.5 Signed informed consent.

1.2 The AD group exclusion criteria: 1.2.1 Sequelae after previous history of severecentral nervous system infection, multiple sclerosis, autoimmune encephalitis,Hashimoto's encephalopathy, etc. 1.2.2 Previous history of instable epilepsy. 1.2.3 Systemic diseases affect thecentral nervous system (CNS), such as abnormal liver and kidney functions. 1.2.4 History of hereditary diseases that affect cognitive function (such asHuntington's disease, Down's syndrome, CADASIL, adrenal leukodystrophy, mitochondrialencephalopathy, etc.). 1.2.5 Infection and immune-related diseases affecting the central nervous system (systemic lupus erythematosus, undertreated HIV infection or a history of CNS syphilisinfection, etc.). 1.2.6 Metabolic and endocrine disorders (requiring new treatment or adjustment ofcurrent treatment for thyroid dysfunction, folate or vitamin B12 deficiency). 1.2.7 Had contraindications for MRI (e.g., pacemakers, stents, claustrophobia.) or didnot cooperate with PET scans. 2. The inclusion and exclusion criteria of post-stroke cognitive observation group. 2.1 The inclusion criteria of post-stroke cognitive observation group: 2.1.1 Aged 40-100-years old (≥ 40 years old, ≤ 100 years old). 2.1.2 Cerebral infarction isdiagnosed according to World Health Organization diagnostic criteria13 and was thefirst symptomatic onset. 2.1.3 The time from onset to enrollment was less than 7 days. 2.1.4 Had adequatehearing, vision and comprehension and verbal expression to complete the cognitiveassessments. 2.1.5 Had at least 3 years of education. 2.1.6 Signed informed consent. 2.2 The exclusion criteria of post-stroke cognitive observation group: 2.2.1 Prior tothe onset of acute infarction had no conditions known to affect cognitive function,such as vascular dementia, dementia with Lewy body dementia, frontotemporal dementia,Parkinson's disease dementia, epilepsy, stroke, hydrocephalus, multiple sclerosis,traumatic brain injuries, genetic disorders affecting cognition, alcoholism,uncontrolled depression or other psychiatric disorders and Alzheimer's disease andIQCODE>3.5. 2.2.2 Sequelae after previous history of severe central nervous system infection,multiple sclerosis, autoimmune encephalitis, Hashimoto's encephalopathy, etc. 2.2.3 Previous history of instable epilepsy. 2.2.4 Systemic diseases affect the CNS,for abnormal liver and kidney functions. 2.2.5 History of hereditary diseases that affect cognitive function (such asHuntington's disease, down syndrome, CADASIL, adrenal leukodystrophy, mitochondrialencephalopathy, etc.). 2.2.6 Infection and immune-related diseases affecting the central nervous system (systemic lupus erythematosus, undertreated HIV infection or a history of CNS syphilisinfection, etc.). 2.2.7 Metabolic and endocrine disorders (requiring new treatment or adjustment ofcurrent treatment for thyroid dysfunction, folate or vitamin B12 deficiency). 2.2.8 Reject or had contraindications for MRI (e.g., pacemakers, stents,claustrophobia.). 3. The inclusion and exclusion criteria of normal cognitive group.

3.1 The inclusion criteria of normal cognitive group: 3.1.1 Aged 40-100 years old (≥ 40years old, ≤ 100 years old). 3.1.2 The patients are cognitively normal and able to live andwork independently.

3.1.3 Had adequate hearing, vision and comprehension and verbal expression to complete thecognitive assessments.

3.1.4 Had at least 3 years of education. 3.1.5 Signed informed consent.

3.2 The exclusion criteria of normal cognitive group: 3.2.1 The patients had no conditionsknown to affect cognitive function, such as vascular dementia, dementia with Lewy bodydementia, frontotemporal dementia, Parkinson's disease, epilepsy, stroke, hydrocephalus,multiple sclerosis, traumatic brain injuries, genetic disorders affecting cognition,alcoholism, uncontrolled depression or other psychiatric disorders, Parkinson's disease,epilepsy or Alzheimer's disease.

3.2.2 Sequelae after previous history of severe central nervous system infection, multiplesclerosis, autoimmune encephalitis, Hashimoto's encephalopathy, etc.

3.2.3 Previous history of instable epilepsy. 3.2.4 Systemic diseases affect the CNS, forabnormal liver and kidney functions.

3.2.5 History of hereditary diseases that affect cognitive function (such as Huntington'sdisease, down syndrome, CADASIL, adrenal leukodystrophy, mitochondrial encephalopathy,etc.).

3.2.6 Infection and immune-related diseases affecting the central nervous system (systemiclupus erythematosus, undertreated HIV infection or a history of CNS syphilis infection,etc.).

3.2.7 Metabolic and endocrine disorders (requiring new treatment or adjustment of currenttreatment for thyroid dysfunction, folate or vitamin B12 deficiency).

3.2.8 Reject or had contraindications for MRI (e.g., pacemakers, stents, claustrophobia.).