Clinical Trial of New Hypothermic Oxygenated Perfusion System Versus Static Cold Storage

Last updated: September 25, 2019
Sponsor: University of Bologna
Overall Status: Active - Recruiting

Phase

N/A

Condition

Primary Biliary Cholangitis

Treatment

N/A

Clinical Study ID

NCT03837197
RF-2016-02364732
  • Ages 18-100
  • All Genders

Study Summary

With the present study the investigators will evaluate the benefit of end-ischemic HOPE on ECD grafts (livers and kidneys) as compared to SCS. Organs will be perfused through a recently developed machine perfusion (MP) device, from the beginning of back-table procedures till implantation, without increasing CIT. The aim of the study will be demonstrating the ability of HOPE to improve graft function and post-operative outcomes of ECD kidney and liver recipients.

Eligibility Criteria

Inclusion

Inclusion Criteria:

  • For kidneys: donor age ≥ 60 years or 50-59 years with two or more of the sequent riskfactors: death due to cerebrovascular accident, history of hypertension, donor serumcreatinine > 1.5 mg/dL, cold ischemia time (CIT) > 20 h;

  • For livers: donors with hemodynamic deterioration, donor age > 65 years, donor bodymass index > 30 kg/m2, serum bilirubin > 3 mg/dl, AST or ALT above three times theupper reference threshold, sodium > 165 mmol/l, intensive care unit (ICU) stay > 7days, steatosis > 40%, CIT > 12 h.

Exclusion

Exclusion Criteria:

  • Donor age ≤18 years;

  • Donors after circulatory death (DCD) will be excluded, because Italian law requires 20minutes of "no touch period" before death declaration, causing prolonged warm ischemiaand subsequent mandatory perfusion of the organ.

  • Split-liver recipients, liver transplantation (LT) for acute liver failure,pre-emptive renal transplant, dual kidney transplantation, and patients withintra-operative surgical complications before the organ implantation.

Study Design

Total Participants: 220
Study Start date:
December 21, 2018
Estimated Completion Date:
December 31, 2021

Study Description

Extended criteria donors (ECD) are widely utilized due to organ shortage, but this may increase the risk of graft dysfunction and of poorer outcomes.

Hypothermic oxygenated perfusion (HOPE) is a recent organ preservation strategy for marginal kidney and liver grafts, which allows to redirect anaerobic metabolism to aerobic metabolism under hypothermic conditions and to protect grafts from oxidative species-related damage; these mechanisms may potentially improve graft function and survival.

Methods This is an open-label, randomized multicenter clinical trial with the aim of comparing HOPE vs. static cold storage (SCS) in ECD kidney and liver transplantation.

In the study protocol - approved by ethics committee - 220 patients (110 liver recipients and 110 kidney recipients) will be enrolled. Livers and kidneys assigned to HOPE will be perfused by machine perfusion with cold Belzer solution (4°-10°C) and with continuous oxygenation (partial pressure of oxygen = 500-600 mmHg). In the control group, livers and kidneys undergoing SCS will be steeped in Celsior or University of Wisconsin Belzer solutions and stored in ice. Using the same perfusion machine for both liver and kidney grafts, organs will be perfused from the start of the back-table procedure until implantation, without increasing cold ischemia time (CIT). For each group the investigators will evaluate clinical outcomes, graft function tests and histologic findings, as well as perfusate and the number of allocated organs.

Connect with a study center

  • Azienda Ospedaliera di Bologna - Policlinico S. Orsola Malpighi

    Bologna, 40138
    Italy

    Active - Recruiting

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