Intravital Microscopy in Human Solid Tumors

Inclusion Criteria:

• Age ≥ 18 years of age

• Eastern Cooperative Oncology Group (ECOG)Performance Status of ≤ 2

• Measurable tumor by direct visualization requiring surgical resection in theoperating room (OR)

• Tumor types of origin include gastric, pancreatic, hepatobiliary, colorectal,sarcoma, brain, or breast cancer that may involve the axillary lymph nodes cancers.Tumors may be primary or metastatic

• Subject must understand the investigational nature of this study and sign anIndependent Ethics Committee/Institutional Review Board approved written informedconsent

• Subject must have a skin prick test pre-operatively (at the time of the preoperativevisit and after signed informed consent for entry into this clinical trial is given)to determine any sensitivity to fluorescein

Exclusion Criteria:

• Uncontrolled intercurrent illness including, but not limited to, ongoing or activeinfection, symptomatic congestive heart failure, unstable angina pectoris, cardiacarrhythmia, or psychiatric illness/social situations

• Renal dysfunction as defined as a glomerular filtration rate (GFR) < 45

• Liver dysfunction as defined by Child-Pugh score > 5, or liver function test (LFT)'s 1.5 x above normal range

• Any known allergy or prior reaction to fluorescein or a positive skin prick test tofluorescein

• Pregnant or nursing female subjects, determined preoperatively with a urinepregnancy test

• Unwilling or unable to follow protocol requirements

• Any condition which in the investigators' opinion deems the patient unsuitable (e.g., abnormal electrocardiography [EKG], including T wave inversion, elevated Twaves, prolonged QRS interval, or conduction blocks) or that requires furtherwork-up (including cardiac echo or stress test)

• Any condition that excludes surgical resection as the standard of care for thepatient