Treatment of Patients With Relapsed or Refractory CD19+ Lymphoid Disease With T Cells Expressing a Third-generation CAR

Inclusion Criteria:

Stratum I/II (Adults):

• Confirmed CD19+ ALL, CLL, DLBCL, FL or MCL in patients ≥ 18 years

• ALL (Ph+ and Ph-): Confirmed CD19+ ALL by cytology and flow cytometry (FACS) AND

• Relapsed or refractory disease (including "molecular relapse" with minimal residualdisease (MRD) levels > 10^-3 at two occasions > 2 weeks apart) with confirmed CD19expression on malignant cells in relapse

• Any relapse after allogeneic stem cell transplantation (alloSCT) (≥ 6 monthsfrom alloSCT at time of CAR T cell infusion) OR

• Any relapse failing to achieve an MRD level of < 10^-3 after ≥ 2 lines oftreatment OR

• Primary refractory as defined by not achieving a complete remission (CR) after ≥ 2 lines of treatment

• CLL/NHL: Confirmed CD19+ CLL/NHL (including CLL, DLBCL, FL or MCL) with

• CLL in need of treatment with:

1. Early relapse (within 2 years) after end of chemoimmunotherapy orchemoimmunotherapy refractoriness plus failure or intolerance of bothBruton's tyrosine kinase Inhibitor (BTKi) and B-cell lymphoma 2 inhibitors (BCL-2i) OR
2. Relapse after alloSCT, ineligible for or refractory to standardinterventions (donor lymphocyte infusions (DLI), CD20 antibodies,chemoimmunotherapy)

• DLBCL with:

1. Refractoriness to a 2nd or later line of chemoimmunotherapy OR
2. Relapse after autologous stem cell transplantation (autoSCT) plusineligibility for alloSCT (including refractoriness to one line of salvagechemoimmunotherapy) OR
3. Relapse after alloSCT

• FL in need of treatment with:

1. Relapse <2 years after chemoimmunotherapy AND ineligibility for or failureof autologous stem cell transplantation (autoSCT) AND ineligibility for orfailure of idelalisib OR
2. Relapse after alloSCT, ineligible for or refractory to standardinterventions (DLI, CD20 antibodies, chemoimmunotherapy)

• MCL with:

1. Relapse after standard first-line therapy AND ineligibility for or failureto BTKi salvage therapy OR
2. Relapse after alloSCT AND ineligibility for or failure to BTKi salvagetherapy

• Measurable disease/MRD at time of enrollment

• Life expectancy ≥ 12 weeks

• Eastern Cooperative Oncology Group (ECOG) performance status ≤ 2 at the time ofscreening

• Adequate organ function:

• Renal function defined as: serum creatinine of ≤ 2 x ULN or estimatedglomerular filtration rate (eGFR) ≥ 30 mL/min/1.73 m^2

• Liver function defined as:

• ALT ≤ 5 times the ULN for the respective age

• Bilirubin ≤ 2.0 mg/dl with the exception of patients with hyperbilirubinemiaexplained by Gilbert-Meulengracht syndrome (may be included if total bilirubinis ≤ 3.0 x ULN and direct bilirubin ≤ 1.5 x ULN) or extrahepatic disease (e.g.chronic hemolytic anemia)

• minimum level of pulmonary reserve defined as ≤ grade 1 dyspnea and pulseoxygenation > 90% on room air

• Hemodynamic stability and left ventricular ejection fraction (LVEF) ≥ 40% asconfirmed by echocardiogram

• Absolute neutrophil count (ANC) ≥ 500/mm3

• Absolute lymphocyte count (ALC) ≥ 100/mm3

• Women of child-bearing potential (defined as all women physiologically capable ofbecoming pregnant) and all male participants must agree to use highly effectivemethods of contraception for one year following CD19.CAR T cell therapy

• Ability to understand the nature of the trial and the trial related procedures

• Written informed consent must be obtained prior to any screening procedures

Stratum III (Children and Adolescents with ALL):

• Age of > 3 years until < 18 years at the time of screening

• CD19+ ALL (Ph+ and Ph-) confirmed by cytology and flow cytometry (FACS) AND

• Relapsed or refractory disease (including "molecular relapse" with polymerase chainreaction (PCR) MRD > 10^-3 at two occasions > 2 weeks apart) with confirmed CD19expression on malignant cells in relapse

• Any relapse after alloSCT (≥ 6 months from alloSCT at time of CAR T cellinfusion) OR

• Any relapse failing to achieve an MRD level of < 10^-3 after ≥ 2 lines oftreatment OR

• Primary refractory as defined by not achieving a CR after ≥ 2 lines oftreatment

• Measurable disease/MRD at time of enrollment

• Life expectancy ≥ 12 weeks

• ECOG performance status ≤ 2 (age ≥ 16 years) or Lansky performance status ≥ 50 (age < 16 years) at the time of screening

• Adequate organ function:

• Renal function defined as serum creatinine-clearance ≥ 30 mL/min/1.73 m^2

• Liver function defined as:

• ALT ≤ 5 times the ULN for the respective age

• Bilirubin ≤ 2.0 mg/dl with the exception of patients with hyperbilirubinemiaexplained by Gilbert-Meulengracht syndrome or extrahepatic disease (e.g.chronic hemolytic anemia)

• minimum level of pulmonary reserve defined as ≤ grade 1 dyspnea and pulseoxygenation > 90% on room air

• Hemodynamic stability and LVEF ≥ 40% or shortening fraction > 29% as confirmedby echocardiogram

• ANC) ≥ 500/mm3

• ALC ≥ 100/mm3

• Women of child-bearing potential (defined as all women physiologically capable ofbecoming pregnant) and postpubertal male participants must agree to use highlyeffective methods of contraception for one year following CD19.CAR T cell therapy

• Written informed consent of the study patient and/or the legal representative mustbe obtained prior to any screening procedures

Exclusion Criteria:

Stratum I/II (Adults):

• The following medications are excluded:

• Immunosuppressive medication with the exception of ≤ 30 mg prednisolone/d orequivalent at the time of CAR T cell transfusion

• Bridging/maintenance therapy including chemo- and immunotherapy must be stopped ≥ 2 weeks prior to leukapheresis, but can be continued between leukapheresisand lymphodepletion

• Intrathecal chemotherapy is possible at any time, but not during lymphodepletionuntil 14 days after CD19.CAR T cell transfusion

• Any DLI must be completed > 6 weeks prior to CD19.CAR T cell infusion

• Florid/acute or chronic Graft-versus-Host disease (GvHD)

• Uncontrolled active hepatitis B or C

• HIV-positivity

• Uncontrolled acute life-threatening bacterial, viral or fungal infection

• Severe concomitant disease (e.g. uncontrolled arterial hypertension, heart failureNew York Heart Association (NYHA) III-IV, uncontrolled diabetes mellitus,uncontrolled hyperlipidemia)

• Unstable angina and/or myocardial infarction within 3 months prior to screening

• Any previous or concurrent malignancy.

The following exceptions do NOT constitute exclusion criteria:

• Adequately treated basal cell or squamous cell carcinoma (adequate wound healing isrequired prior to study entry)

• In situ carcinoma of the cervix or breast, treated curatively without evidence ofrecurrence ≥ 3 years prior to the study

• CLL or FL transformed into an aggressive B cell lymphoma

• A primary malignancy which is in complete remission for ≥ 5 years

• Pregnant or nursing (lactating) women

• Intolerance to the excipients of the cell product

• Active central nervous System (CNS) involvement in ALL patient at the time ofscreening is not an exclusion criterion, but patients with CNS 3 status atclinical screening (d-14) are not eligible for CD19.CAR T cell transfusion

• Participation in another clinical trial at the time of screening

Stratum III (Children and Adolescents with ALL):

• The following medications are excluded:

• immunosuppressive medication with the exception of < 0.5 mg/d*kg body weight (BW) prednisolone-equivalent at the time of CD19.CAR T cell transfusion

• Bridging/Maintenance therapy including chemo- and immunotherapy must be stopped ≥ 2 weeks prior to leukapheresis, but can be continued between leukapheresisand lymphodepletion

• Intrathecal chemotherapy is possible at any time, but not during lymphodepletionuntil 14 days after CD19.CAR T cell transfusion

• Any DLI must be completed > 6 weeks prior to CD19.CAR T cell infusion

• Florid/acute or chronic GvHD

• Uncontrolled active hepatitis B or C

• HIV-positivity

• Uncontrolled acute life-threatening bacterial, viral or fungal infection

• Severe concomitant disease (e.g. any life-limiting genetic disorder). Patients withDown Syndrome will not be excluded.

• Any previous or concurrent malignancy.

The following exceptions do not constitute exclusion criteria:

• Lymphoblastic lymphoma transformed into a CD19+ acute lymphoblastic leukemia

• A primary malignancy which is in complete remission for ≥ 5 years

• Pregnant or nursing (lactating) women

• Intolerance to the excipients of the cell product

• Active CNS involvement at the time of screening is not an exclusion criterion,but patients with CNS 3 status at clinical screening (d-14) are not eligiblefor CD19.CAR T cell transfusion

• Participation in another clinical trial at the time of screening