Effect of Fecal Microbiota Transplantation in Irritable Bowel Syndrome

Inclusion Criteria:

• Fulfilled Rome III diagnostic criteria for IBS

• Moderate-severe disease activity (IBS-Symptom Severity Score ≥175)

• Able to read and speak Danish

• Normal colonoscopy at age ≥ 40 years (performed within 1 year) or blood in stool

Exclusion Criteria:

• Other chronic gastrointestinal disease

• Positive fecal sample with enteropathogenic microorganisms or Clostridium difficile

• Positive screening for HIV, Hepatitis B or HCV antibody

• Surgical interventions in the gastrointestinal region (except for appendectomy, herniarepair, cholecystectomy, and gynecological and urological procedures)

• Psychiatric disorder

• Fecal calprotectin ≥ 50 mg/kg

• Abuse of alcohol or drugs

• Medications other than birth control pills, hormone supplements, allergies and asthmaagents, blood pressure and cholesterol lowering agents, proton pump inhibitors andnon-prescription medicines

• Abnormal screening biochemistry

• Abnormal colonoscopy findings

• Pregnant, planned pregnancy or breastfeeding females

• Ingestion of probiotics or antibiotics < 8 weeks before the inclusion Inclusion criteria for donors

• Age between 18-45 years

• Past and current healthy

• Normal weight (BMI between 18,5-24,9 kg/m2)

• Normal bowel movements (defined as 1-2 per day and type 3-4 at Bristol Stool FormScale)

• No medication consumption Exclusion criteria for donors

• Known or high risk of infectious diseases such as HIV, hepatitis A, B or C

• Positive stool sample for C. difficile toxin, parasites or other pathogens

• Antibiotic treatment in the past 6 months

• Abuse of alcohol or drugs

• Smoking

• Tattoo or body piercing within the last 6 months

• Allergy, asthma or eczema

• Family history of gastrointestinal diseases

• Participation in high-risk sexual behaviors

• Born by Caesarean section