Investigating SX-682 in Combination With Apalutamide in Metastatic Castration-resistant Prostate Cancer

Inclusion Criteria:

1. Written informed consent and be capable of cooperating with treatment.

2. Age ≥ 18 years.

3. Histologically or biochemically confirmed adenocarcinoma of the prostate and withtumour tissue accessible for research analysis for this trial. Patients who have nohistological diagnosis must be willing to undergo a biopsy to prove prostateadenocarcinoma.

4. Patients recruited to phase 1 dose escalation cohorts must have biopsiable diseaseand consent to mandatory pre- and post-treatment biopsies (baseline and on Cycle 2Day 1).

5. Metastatic castration-resistant prostate cancer.

6. All patients must have documented resistance to 1 prior next generation antiandrogentherapy (NAAT) defined as: For phase 1 and phase 2 Cohorts: Patients who have progressed after either enzalutamide, Apalutamide or darolutamide (having received a minimum of 12-weeks of enzalutamide, Apalutamide or darolutamide)will enter phase 1 or phase 2 cohorts directly. Patients that have previouslyreceived abiraterone but not an AR antagonist should receive a lead-in withApalutamide on trial and receive the combination on progression through the lead-in.

7. Documented prostate cancer progression as assessed by the investigator with RECISTv1.1 and PCWG3 criteria (Section 3.5) with at least two of the following criteria:

8. Progression of soft tissue/visceral disease by RECIST v1.1 and/or,

9. Progression of bone disease by PCWG3 bone scan criteria and/or,

10. Progression of PSA by PCWG3 PSA criteria.

11. PSA ≥ 10ng/ml.

12. Received prior castration by orchiectomy and/or ongoing luteinizing hormonereleasing hormone agonist treatment.

13. Ongoing androgen deprivation with serum testosterone < 50 ng/dL (< 1.7 nM).

14. Eastern Cooperative Oncology Group (ECOG) Performance Status of ≤2.

15. Documented willingness to use an effective means of contraception whileparticipating in the study and for 6 months post last treatment dose.

16. Able to swallow the study drug.

17. All efforts should be made to discontinue steroid usage but up-to 5mg BDprednisolone (or equivalent) will be allowed.

18. Haematological and biochemical indices within the ranges shown below. Thesemeasurements must be performed within one week (Day -7 to Day 1) before the patientgoes on trial.

Haemoglobin (Hb) ≥ 9.0 g/dL Absolute neutrophil count ≥ 1.5 x 109/L Platelet count ≥ 100 x 109/L WBC ≥ 3.0 x 109/L Calculated creatinine clearance ≥ 50 mL/min (uncorrected value) Serum bilirubin ≤ 1.5 x upper limit of normal (ULN) unless documented Gilbert's disease., in which case ≤ 3 x ULN is permissible Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ≤ 2.5 x (ULN) unless raised due to known metastatic liver disease in which case ≤ 5 x ULN is permissible

Exclusion Criteria:

1. Surgery, chemotherapy, or other anti-cancer therapy within 4 weeks prior to trialentry/randomisation into the study (with the exception of abiraterone, enzalutamide,Apalutamide or darolutamide). Any other therapy for prostate cancer, other thangonadotropin releasing hormone analogue therapy, such as progesterone,medroxyprogesterone, progestins or 5-alpha reductase inhibitors, must bediscontinued at least 2 weeks before the first dose of the study drug.

2. Participation in another interventional clinical trial of an IMP within 4 weeksprior to trial entry. Participation in trials of licensed medications is allowedprovided the medication is not a prohibited concomitant medication.

3. Prior limited field radiotherapy within 2 weeks and wide field radiotherapy within 4weeks prior to trial entry.

4. Clinical and/or biochemical evidence of hyperaldosteronism or hypopituitarism.

5. History of seizures or other predisposing factors including, but not limited to,underlying brain injury, stroke, primary brain tumours, brain metastases andleptomeningeal disease, or alcoholism.

6. Malabsorption syndrome or other condition that would interfere with enteralabsorption.

7. Any of the following cardiac criteria:

• QTcF interval > 470 msec.

• Clinically important abnormalities including rhythm, conduction, orelectrocardiogram (ECG) changes (left bundle branch block, third degree heartblock).

• Factors predisposing to QT prolongation including heart failure, hypokalaemia,congenital long QT syndrome, family history of prolonged QT syndrome,unexplained sudden death (under 40) and concomitant medications known toprolong QT interval.

• Coronary artery bypass, angioplasty, vascular stent, myocardial infarction,angina, congestive heart failure (NYHA ≥ grade 2) i or transient ischaemicattack) in the last 6 months (see appendix 4 for NYHA scale).

• Uncontrolled hypotension (systolic blood pressure < 90mmHg).

• Uncontrolled hypertension on optimal medical management.

8. Clinically significant history of liver disease (Child-Pugh B or C, viral or otherhepatitis, current alcohol abuse or cirrhosis).

9. Any other finding giving reasonable suspicion of a disease or condition thatcontraindicates the use of an investigational drug or that may affect interpretationof the results or renders the patients at high risk from treatment complications,e.g., patients with a hypersensitivity to the active substance or any of theexcipients.

10. Malignancy other than prostate cancer within 5 years of trial entry except foradequately treated basal cell carcinoma.

11. Unresolved significant toxicity from prior therapy (except alopecia and grade 1peripheral neuropathy).

12. Inability to comply with study and follow-up procedures.

13. Predominantly small cell or neuroendocrine differentiated (> 20% of cells) prostatecancer.

14. Immunocompromised patients.

15. Active or uncontrolled autoimmune disease requiring corticosteroid therapy.

16. History of thromboembolic disease within 12 months of commencement of trial.

17. At high-risk because of non-malignant systemic disease including active infectionand any serious concurrent illness.

18. Any known intolerance to Apalutamide, SX-682, or to any constituents.

19. Symptoms of COVID-19 and/or documented COVID-19 infection.

20. Is taking any of the following prohibited medications:

• Aminophylline/theophylline

• Atypical antipsychotics (eg, clozapine, olanzapine, risperidone, ziprasidone)

• Buproprion

• Lithium

• Meperidine and pethidine

• Phenothiazine antipsychotics (eg, chlorpromazine, mesoridazine, thioridazine)

• Tricyclic and tetracyclic antidepressants (eg, amitriptyline, desipramine,doxepin, imipramine, maprotiline, mirtazapine

• Warfarin or coumarin-like anticoagulants

21. History of previous non-infectious pneumonitis requiring steroid treatment, oractive non-infectious pneumonitis.

22. History of previous severe drug induced severe cutaneous reaction including but notlimited to Steven-Johnson's syndrome/toxic epidermal necrolysis, drug reaction witheosinophilia and systemic symptoms (DRESS).