Investigating the Bactericidal Effect of Probiotics on Chromogenic Bacteria in Children An In Vivo and In Vitro Study

Last updated: April 13, 2025
Sponsor: Ain Shams University
Overall Status: Completed

Phase

4

Condition

N/A

Treatment

Probiotic with Prebiotic

Clinical Study ID

NCT06834815
FDASU-Rec IR102420
  • Ages 6-14
  • All Genders
  • Accepts Healthy Volunteers

Study Summary

To investigate the bactericidal effect of probiotics (Streptococcus salivarius M18 and Lactobacillus reuteri) on two types of bacteria (Aggregatibacter actinomycetemcomitans and Actinomyces naeslundii) causing black stains of teeth in vitro.

Eligibility Criteria

Inclusion

Inclusion Criteria:

  • Patients with black stains on teeth scored via modified Lobene index scores 1-3.

  • Medically free patients

Exclusion

Exclusion Criteria:

  • Stains other than black.

  • Patients on antibiotic treatment or any other medication for the last 2 weeks.

  • Patients allergic to prophylaxis powder.

  • Parents refusing to participate

Study Design

Total Participants: 9
Treatment Group(s): 1
Primary Treatment: Probiotic with Prebiotic
Phase: 4
Study Start date:
November 05, 2024
Estimated Completion Date:
March 01, 2025

Study Description

In vitro:

An unstimulated salivary sample will be taken from patients having black stains. The two types of bacteria of each condition will be cultured, amplified, and measured in the laboratory from the saliva of these patients. After that, one type of probiotics will be applied to these bacteria separately and the same bacterial measurement will be done to evaluate the bactericidal effect of the probiotics on these bacteria, aiming to find the minimal inhibitory concentration of the probiotics.

Connect with a study center

  • faculty of dentistry .Ain shams university

    Cairo, 11566
    Egypt

    Site Not Available

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