GLYLO Supplement Pilot Trial on Glycation and Aging in Postmenopausal Women

Inclusion Criteria:

1. Adults identified as female at birth with ovaries present (self-report)

2. Post menopause >1y since last menses (self-report)

3. Aged 45 - 65 y

4. BMI ≥ 27 (screening measurement). Participants may weigh themselves at home for twodays and share with the study team if their weight at the screening visit results inthem being slightly under the BMI cut-off.

5. HbA1c 5.7- 6.4% (screening measurement)

6. Able to read and speak English well enough to provide informed consent andunderstand instructions.

7. Able to attend in-person visits at The Buck Institute

Exclusion Criteria:

1. Not identified as female at birth (self-report)

2. Surgical menopause (self-report)

3. Hysterectomy and/or ovariectomy (self-report)

4. Receiving hormone replacement therapy (HRT) (self-report).

5. Currently prescribed or received weight loss medications within the past 6 months,or currently enrolled in a defined weight loss program. Weight must be stable (> 4%)within the last 3 months.

6. Regular use of GLYLO, or regular use of a supplement containing any of theingredients in GLYLO, within the last 3 months.

7. Diabetes, T1DM or T2DM (self-report and screening tests): Treatment with anyhypoglycemic agents (self-report), fasting glucose >125 mg/dL (screening test; mayreassess once), current use of hypoglycemic drugs for non-diabetic reasons (self-report).

8. Elevated blood pressure readings (screening test): Resting Systolic Blood Pressure (SBP) ≥150 mmHg or resting Diastolic Blood Pressure (DBP) ≥90 mmHg. Participants mayprovide home readings for two days if they do not meet this criterion at thescreening visit.

9. Psychotropic and/or other medications known to significantly impact weight unless ona stable dose for ≥ 6 months (self-report).

10. Liver enzyme tests (alanine transaminase, aspartate transaminase) (screening test): >2 times the laboratory upper limit of normal. Reassessment during screening may beallowed under some conditions (e.g., recent use of acetaminophen).

11. Immunosuppressive disorders, taking immunosuppressive medications (including oralprednisone >10mg/day and biological immunosuppressants), or receiving chemotherapy.

12. Active gastrointestinal bleeding, or active bleeding diathesis (or resolved within 6months prior to randomization) (self-report)

13. Active peptic ulcer disease (or resolved within 6 months prior to randomization) (self-report)

14. Active malignancy (or resolved within 6 months prior to randomization), exceptnon-melanoma skin cancer not undergoing treatment (self-report).

15. Active infection (or resolved within 1 month prior to randomization) (self-report)

16. Allergy or hypersensitivity to any component of the supplement (self-report)

17. History of hyperthyroidism or current use of thyroid hormones (self-report), currentabnormal thyroid function (blood test at screening).

18. Cognitive status: Unable to provide informed consent to participate in and safelycomplete the protocol, as based on the judgment of the investigators (screeningvisit)

19. Psychiatric status: any condition that might affect the ability to comply with theprotocol in the opinion of the Clinical Investigator or Medical Officer (screeningvisit)

20. Active eating disorders (self-report).

21. Active diagnosis of Gout (self-report)

22. Any change to prescription medications within 3 months prior to randomization thatare judged by the study physician to impact the results of the study (self-report)

23. No overnight hospitalization within 1 month prior to randomization (self-report)

24. The presence of a condition or abnormality that in the opinion of the Investigatoror Medical Officer would compromise the safety of the patient or the quality of thedata