This study's research design is a pilot single-arm intervention trial. Data collection
will occur three times (baseline, midpoint, and post-intervention) over the course of the
8 week intervention period. Data collection will take place on Nova Southeastern
University Campus (3301 S University Dr, Davie, FL 33328). Eligible participants will
undergo an 8-week supplementation regimen with Life Extension® Extend Release Magnesium,
containing a 250 mg/capsule blend of ZümXR® magnesium oxide and magnesium citrate. The
supplement will be distributed to participants at baseline and midpoint measurement
assessments. Dietary intake will be monitored at least 3 days of the week for the 8 week
intervention period using the MyFitnessPal app. Participants will be instructed not to
change their eating habits for the duration of the intervention. Outside of their usual
physical activity training, participants will be instructed not to change their physical
activity habits for the duration of the intervention. Body composition will be assessed
at baseline, midpoint, and post-intervention using Bioelectrical Impedance Analysis
(BIA). Participants will be instructed to wear light clothing (with no zippers, belts, or
buttons), remove all jewelry before testing, not consume any food 3-4 hours before
testing, not drink caffeine on the day of testing, and hydrate adequately prior to
testing. Demographic information (age, sex, and physical activity status) will be
assessed via a simple questionnaire at baseline.
Order of events:
Distribute recruitment through flyers at NSU Main Campus (Davie).
Screening process via demographic survey to determine qualification based on
participant inclusion/exclusion criteria.Sign consent forms, explain procedures and
study outcomes to participants.
Baseline anthropometric measurements (Bioelectrical Impedance Analysis (BIA) [InBody
270; InBody Co., Ltd. (Seoul, Korea)], height, and weight) and diet analysis
(MyFitnessPal Nutrition Tracking App).
Distribute supplements for the first 4 weeks of intervention (Life Extension Extend
Release Magnesium, 28 capsules/participant).
Bi-weekly follow ups with participants via phone (2 follow ups).
Midpoint anthropometric measurements (BIA [InBody 270; InBody Co., Ltd. (Seoul,
Korea)] and diet analysis (MyFitnessPal Nutrition Tracking App). Perform capsule
counting.
Distribute supplements for the last 4 weeks of intervention (Life Extension
Magnesium Caps, 28 capsules/participant).
Bi-weekly follow ups with participants via phone (2 follow ups).
Final anthropometric measurements (BIA [InBody 270; InBody Co., Ltd. (Seoul, Korea)]
and diet analysis (MyFitnessPal Nutrition Tracking App). Perform capsule counting.
Data collection:
Height and weight will be collected at baseline, midpoint, and post-intervention visits
using weight scale and stadiometer. BMI will be calculated using height and weight. Body
composition will be assessed using a multi-frequency Bioelectrical Impedance Analysis
(BIA) device [InBody 270; InBody Co., Ltd. (Seoul, Korea)].
Participants will be required to keep a food diary for at least 3 days per week using the
MyFitnessPal (MFP) Nutrition Tracking Smartphone Application. Similar software has been
shown to produce results comparable to other, more time-intensive methods of dietary data
collection such as 24-hour recalls. Participants' diets will be analyzed during the
baseline and midpoint assessment visits using the MyFitnessPal (MFP) Nutrition Tracking
App. Diet analyses will be conducted to determine how much dietary magnesium and protein
participants are consuming in their normal diet, as both factors may affect LBM accrual.
