A Diagnostic Test to Evaluate Cancer Risk Before Surgery in Women with an Ovarian Mass

Inclusion Criteria:

• female patients => 18 years of age at the time of consent

• Can provide written informed consent

• Have an ovarian cyst or adnexal mass (simple, complex, or solid ovarian/pelvic mass)identified through imaging examination (e.g. transvaginal ultrasound - TVU) thatrequires surgery, but have not yet undergone this surgery

Exclusion Criteria:

• Any prior confirmed diagnosis of, or treatment for, ovarian cancer

• Any prior surgery resulting in removal of both ovaries

• Prior history of gynecological malignancy (within last 2 years)

• Prior history of melanoma (within last 2 years)

• Prior treatment (within 12 months of sample collection) with chemotherapy,radiotherapy or immunotherapy

• Immune-compromised. Definition: those whose weakened immune system may render themmore susceptible to infection, illness, or complications. These may include patientswith immune-suppressive conditions (e.g. HIV/AIDS), patients undergoing immuno-,chemo- or radio-therapy (within the last 12 months) for any reason, receiving immunesuppressive therapies (e.g. transplant recipients, individuals with known autoimmunedisease), or with a primary immunodeficiency disorder (e.g. Lupus).

• Pregnant currently or within the last 3 months based on participant self-report