Myeloablative Conditioning Orca-T & Allogeneic Donor-Derived CD19/CD22-CAR TCells in B-Cell ALL

Patient Inclusion Criteria:

• Subjects in CR must have a history of chemotherapy refractory disease defined asprogression or stable disease after one line of chemotherapy, or relapsed diseaseafter achieving prior CR OR must have other high risk ALL features including: CRLF2rearrangement, Ph-like phenotype, MLL/KMT2a rearrangement, or hypodiploid karyotype.

• Subjects with persistent or relapsed minimal residual disease (MRD) (by flowcytometry, PCR, FISH, or next generation sequencing) require verification of MRD inthe peripheral blood or bone marrow on two occasions at least 2 weeks apart.

• Subjects with active ALL (defined as >=5% bone marrow blasts, circulating blasts, orextramedullary disease) are eligible.

• Age ≥ 18 and ≤ 65 years (i.e., from age 18 to < 66 years old) at the time ofenrollment

• Eastern cooperative oncology group (ECOG) performance status of 0, 1, or 2; orKarnofsky ≥ 60%

• CD19 expression is required any time since diagnosis. CD19 expression may bedetected by immunohistochemistry or by flow cytometry. The choice of whether to useflow cytometry or immunohistochemistry will be determined by what is the most easilyavailable tissue sample in each subject. In general, immunohistochemistry will beused for lymph node biopsies, flow cytometry will be used for peripheral blood andbone marrow samples. Patients receiving prior CD19 CAR T cell or blinatumomab areeligible if there is no documented history of CD19 negativity on the malignantcells.

• Subjects must have an HLA matched related donor willing to undergo unstimulatedapheresis for T cell collection for CAR T cell generation followed by GCSF mobilizedapheresis for HSC/Treg graft.

• Matched related donor who is an 8/8 match for HLA-A, -B, -C, and -DRB1, all typedusing DNA-based high-resolution methods

• Subjects must have adequate organ function measured by:

• Cardiac: Cardiac ejection fraction at rest ≥ 45%

• Hepatic:

• Total bilirubin < 2 times upper limit of normal (ULN) (patients with Gilbert'ssyndrome may be included where hemolysis has been excluded and with approval ofthe study PI)

• ALT/AST <= 3 times ULN

• Renal: Calculated creatinine clearance ≥ 50 mL/min or serum creatinine < 2.0mg/dL

• Pulmonary: Diffusing capacity of the lung for carbon monoxide (DLCO) (adjustedfor hemoglobin) ≥ 50%

• CNS: Subjects with CNS involvement are eligible as long as there are no overtsigns or symptoms that in the evaluation of the investigator would mask orinterfere with the neurological assessment of toxicity.

• Negative serum or urine beta-HCG test in females of childbearing potential within 3weeks of registration

• Subjects of childbearing or child fathering potential must be willing to practicebirth control from the time of enrollment on this study and for twelve (12) monthsafter receiving the preparative conditioning regimen.

• Must be able to give informed consent. Legal authorized representative (LAR) ispermitted if subject is cognitively able to provide verbal assent.

Donor Inclusion Criteria

• Age ≥ 18 and ≤ 75 years at time of enrollment

• Karnofsky performance status of ≥ 70% defined by institutional standards

• Willing to donate for two separate apheresis collections (T cells and PBSC)

• Matched related donor who is an 8/8 match for HLA-A, -B, -C, and -DRB1, all typedusing DNA-based high-resolution methods

• Negative serum or urine beta-HCG test in females of childbearing potential within 2weeks of first apheresis

• Seronegative for HIV-1 RNA PCR; HIV 1 and HIV 2 Ab (antibody); HTLV-1 and HTLV-2 Ab;PCR negative or sAg (surface antigen) negative for hepatitis B; negative for theTreponema pallidum antibody Syphilis screen; and negative for HIV-1 and hepatitis Cby nucleic acid testing (NAT) within 40 days of donor apheresis procedures.

• In the case that T pallidum antibody tests are positive, donors must:

• Be evaluated and show no evidence of syphilis infection of any stage byphysical exam and history

• Have completed effective antibiotic therapy to treat syphilis

• Have a documented negative non-treponemal test (such as RPR) or in the case ofa positive non-treponemal test must be evaluated by an infectious diseaseexpert to evaluate for alternative causes of test positivity and confirm noevidence of active syphilitic disease

Patient Exclusion Criteria:

• History of other malignancy unless disease free for at least 3 years. At thediscretion of the Principal Investigator, subjects in remission for 1-2 years priorto enrollment may be deemed eligible after considering the nature of othermalignancy, likelihood of recurrence for one year following therapy, and impact ofprior treatment on risk of CD19/CD22-CAR T cells and Treg graft. Subjects inremission <1 year are not eligible. The following exceptions apply:

• Nonmelanoma skin cancer or carcinoma in situ (e.g. cervix, bladder, breast) iseligible.

• Hormonal therapy in subjects in remission >1 year will be allowed.

• Patients who have undergone a prior allogeneic or autologous stem cell transplant.

• Recipient positive anti-donor HLA antibodies against a mismatched allele in theselected donor determined by either:

• a positive crossmatch test of any titer (by complement-dependent cytotoxicityor flow cytometric testing), or

• the presence of anti-donor HLA antibody to any of the following HLA loci:HLA-A, -B, -C, -DRB1, -DQB1, -DQA1, -DPB1, or -DPA1, with mean fluorescenceintensity (MFI) >1000 by solid phase immunoassay

• Presence of fungal, bacterial, viral, or other infection that is uncontrolled.Simple UTI and uncomplicated bacterial pharyngitis are permitted if responding toactive treatment.

• Known history of infection with any of the following:

• HIV

• Hepatitis B (HBsAg positive) **

• Hepatitis C virus (anti HCV positive) **

** A history of hepatitis B or hepatitis C is permitted if the viral load isundetectable per quantitative PCR and/or nucleic acid testing.

• Currently receiving corticosteroids or other immunosuppressive therapy. Topicalcorticosteroids or oral systemic corticosteroid doses less than or equal to 10mg/day are allowed.

• Planned pharmaceutical in vivo or ex vivo T cell depletion, e.g., post-transplantcyclophosphamide (Cy), peri-transplant anti-thymocyte globulin (ATG), oralemtuzumab. For patients that have previously been exposed to a T cell-depletingagent, a 5-half-life washout of the agent must occur prior to planned Transplant Day

• Hyperleukocytosis (≥ 50,000 blasts/μL) or rapidly progressive disease that in theestimation of the investigator and sponsor would compromise ability to completestudy therapy.

• History of myocardial infarction, cardiac angioplasty or stenting, unstable angina,or other clinically significant cardiac disease within 12 months of enrollment.

• Pregnant or breast feeding

• Patients with known autoimmune disease requiring the use of systemicimmunosuppressive therapy within the last year

• Presence of a seizure disorder, cerebrovascular ischemia/hemorrhage, dementia,cerebellar disease, or any autoimmune disease with CNS involvement that in thejudgment of the investigator may impair the ability to evaluate neurotoxicity.

• Any medical condition that in the judgement of the investigator is likely tointerfere with assessment of safety or efficacy of study treatment

Donor Exclusion Criteria

• Evidence of active infection

• Seropositive for HIV-1 or -2, HTLV-1 or -2

• Positive PCR test results indicating acute or chronic HBV infection. Patients withisolated HBV core antibody positivity will not be excluded. Donors whose HBVinfection status cannot be determined conclusively by serologic test results (www.cdc.gov/hepatitis/hbv/pdfs/serologicchartv8.pdf) must be negative for HBV byPCR to be eligible for study participation.

• Potential for Zika virus infection as defined as any of the following:

• Medical diagnosis of Zika virus infection in the past 6 months

• Residence in, or travel to, an area with active Zika virus transmission withinthe past 6 months.

• Unprotected sex within the past 6 months with a person who is known to haveeither of the risk factors listed above (donor exclusion criterion 5.a or 5.b)

• Donors determined to be ineligible based on the results of Zika virus screening maybe determined to be eligible if: o The donor has no signs or symptoms consistent with active Zika virus infection and o Either of the following is true: i. The donor is a first-degree or second-degreeblood relative of the recipient ii. Urgent medical need, meaning no comparable humancell product is available and the recipient is likely to suffer death or seriousmorbidity without the human cell product, as attested by the Investigator.

• Pregnant or breastfeeding female

• Medical, physical, or psychological reason that would place the donor at increasedrisk for complications from growth factor or leukapheresis.