Exploration of Differences in Metabolite Concentrations by 7Teslas NMR Spectroscopy in Striatum and Subthalamic Nuclei in de Novo Parkinsonian Patients and Control Subjects

Last updated: February 26, 2026
Sponsor: University Hospital, Clermont-Ferrand
Overall Status: Active - Recruiting

Phase

N/A

Condition

Parkinson's Disease

Dyskinesias

Treatment

specific MRI Acquisition (NMR spectroscopy) at 7T

Clinical Study ID

NCT04735172
AOI 2020 DURIF (METABO-NGC-7T)
2020-A03237-32
  • Ages 18-75
  • All Genders
  • Accepts Healthy Volunteers

Study Summary

Initially, the exploration of brain metabolism by Nuclear Magnetic Resonance Spectroscopy (MRS) of the high magnetic field proton (1H) (11.7T) applied to acute and chronic animal models of Parkinson's disease (PD) showed glutamatergic hyperactivity within the striatum, one of the components of the basal ganglia. Interestingly, acute administration of L-dopa and acute, subchronic and chronic deep brain stimulation of the subthalamic nucleus (STN) normalizes these neurochemical profiles. Investigators also show an increase in glutamate levels in the STN ipsilateral to the substantia nigra pars compacta (SNpc) damaged by the neurotoxin, expected phenomenon, but also and surprisingly in the STN controlateral to the lesion. A degeneration of dopaminergic neurons is also observed in the controlateral SNpc at the lesion suggesting that the hyperglutamatergy of the controlateral STN to the lesion could promote neuronal death in the SNpc and thus participate in the progression and lateralization of the PD.

Using 3T MRS in PD patients, as in other studies in humans, investigators do not see changes in glutamate and glutamine levels in the putamen of Parkinsonian patients. This difference between animal and human studies can be explained:

  1. by the different rate of progression between PD in humans and animal models with plasticity phenomena limiting glutamatergic hyperactivity,

  2. by the effect of treatment in PD masking changes in glutamate metabolism,

  3. by limiting sensitivity in the detection of metabolites (Glutamate, glutamine, GABA) at 3T.

The 7T 1H MRS improves the dispersion of chemical shifts of the metabolites studied, increases the sensitivity of the measurement, makes it possible to select regions of interest of smaller volumes (1 cm3) and thus limits the magnetic susceptibility effects that degrade the quality of the measured signal. This makes it possible to reliably separate glutamate and glutamine peaks.

In this context, investigators propose to study the metabolic changes in a homogeneous group of de novo Parkinsonian patients, naive to any treatment intended to replace the missing dopamine. The gain in spatial resolution, contrast and signal will allow better characterization of localized anomalies in small-volume structures such as basal ganglia, putamen and STN.

Eligibility Criteria

Inclusion

Inclusion Criteria:

Patients will be included:

  • suffering from idiopathic Parkinson's disease according to UKPDSBB criteria (Gibb &Lees, 1988; Hughes et al., 1992),

  • the stage of the disease is I-II according to the Hoehn and Yahr scale,

  • which do not receive dopaminergic treatment,

  • duration of disease development 5 years,

  • without major cognitive impairment (Moca > 24)

  • men or women aged 18 to 75,

  • having understood and signed the informed consent form,

  • members of a social security scheme.

Controls:

  • subjects male or female aged 18 -75 years

  • subjects affiliated to a social security scheme.

  • volunteers who have given their written consent.

Exclusion

Exclusion Criteria:

Patients will be excluded:

  • having a severe tremor (> 3 for a trembling sub-item of UPDRS 3) making the MRexamination impossible,

  • patients with "contra-indications" to an MRI exam (without administration of agadolinium chelate): presence of metal parts in the body (electronic devices such asa pacemaker, a neurostimulator, a cochlear implant, prostheses, etc.),claustrophobia,

  • taking any treatment that may interact with brain concentrations ofneurotransmitters, such as all psychotropic drugs and in particular antidepressants,neuroleptics, benzodiazepines, antiepileptics,

  • pregnant women,

  • treated by deep brain neurostimulation,

  • patients under guardianship or guardianship or protection of justice,

  • patients who are excluded from another study.

Controls:

  • persons suffering from progressive neurological and psychiatric pathology,

  • persons with "contra-indications" to an MRI examination (without administration of agadolinium chelate): presence of metal parts in the body (electronic devices such asa pacemaker, a neurostimulator, a cochlear implant, prostheses, etc.),claustrophobia,

  • taking any treatment that may interact with brain concentrations ofneurotransmitters, such as: all psychotropic drugs and in particularantidepressants, neuroleptics, benzodiazepines, antiepileptics,

  • pregnant women,

  • persons under guardianship or trusteeship or protection of justice,

  • people who are excluded from another study.

Study Design

Total Participants: 44
Treatment Group(s): 1
Primary Treatment: specific MRI Acquisition (NMR spectroscopy) at 7T
Phase:
Study Start date:
April 07, 2022
Estimated Completion Date:
August 31, 2029

Study Description

The project presented is an open and controlled exploratory prospective study, assessing metabolic concentrations in putamen and NST left and right of treatment-naïve de novo Parkinsonian patients compared to healthy subjects. Patients will be included consecutively following the screening. Since this study is cross-sectional, a single visit will be made. De novo PD patients will be pre-selected in the departments of Neurology of the CHU of Clermont-Ferrand and Poitiers.

The inclusion visit will be carried out during a consultation during their usual follow-up in the departments of Neurology of the CHU of Clermont-Ferrand and Poitiers.

The following data will be noted: age, sex, level of study, duration of disease progression, ongoing treatments, medical history.

Patients verifying the inclusion and exclusion criteria will be definitively included and their consents will be collected.

De novo PD patients will undergo their NMR examination in the department of radiology, 7T MRI at the Poitiers hospital.

Patients will report to MRI and the following measurements will be performed:

  • measurement of motor disorders by the UPDRS scale, the Hoehn and Yahr and Schwab and England score;

  • acquisition of NMR images and spectra. The experimental time will be 30 minutes for the UPDRS scale and 30 minutes for the NMR exam.

Recruitment of the subjects in the control group will be carried out in the patients' family and in that of the staff of the departments of neurology and radiology.

They will be matched to de novo PD patients according to age, sex and level of education. In practice, after pre-screening the control subjects, they will be informed about the protocol, their consent will be collected. The following data will also be noted: age, sex, duration and level of study.

After verification of the inclusion and exclusion criteria, the witnesses will take the NMR examination in the Radiological Department, 7T MRI at the Poitiers University Hospital Hospital.

Connect with a study center

  • Chu Clermont Ferrand

    Clermont-Ferrand, 63003
    France

    Site Not Available

  • Chu Clermont Ferrand

    Clermont-Ferrand 3024635, 63003
    France

    Site Not Available

  • CHU Poitiers

    Poitiers,
    France

    Site Not Available

  • CHU Poitiers

    Poitiers 2986495,
    France

    Active - Recruiting

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