The Impact of Lifestyle on Sperm Telomere Length and Telomerase Activity in Men Undergoing IVF Treatments

Last updated: June 22, 2022
Sponsor: Hillel Yaffe Medical Center
Overall Status: Active - Recruiting

Phase

N/A

Condition

Infertility

Men's Health

Hypogonadism

Treatment

N/A

Clinical Study ID

NCT04158765
0120-18-HYMC
  • Ages 18-60
  • Male
  • Accepts Healthy Volunteers

Study Summary

This aim of this study is to determine the impact of lifestyle on telomere length and telomerase enzyme activity on sperm cells of men undergoing IVF treatments.

Eligibility Criteria

Inclusion

Inclusion Criteria:

  • Infertile couple

Exclusion

Exclusion Criteria:

  • Chronic disease
  • Azoospermia

Study Design

Total Participants: 100
Study Start date:
November 20, 2019
Estimated Completion Date:
January 31, 2023

Study Description

Following approval of the study by the Hospital Ethical Committee (Helsinki), semen samples and blood will be collected from infertile individuals referred to our IVF unit at Hillel Yaffe Medical Center. Informed written consent will be obtained from each participant. Standard semen analysis will be carried out according to World Health Organization protocol. An aliquot of semen sample will be used for assessment of sperm motility, concentration, and morphology by using computer-aided sperm analysis (CASA) and the remaining sample will be used for assessment of sperm telomere lengths and telomerase activity. Genomic DNA will be extracted from the remaining washed sperm and peripheral blood. Sperm telomere length will be determined by real-time polymerase chain reaction (qRT-PCR) and telomerase activity will be determined by TRAP assay (Telomeric Repeat Amplification Protocol).

Connect with a study center

  • Hille Yaffe Medical Center

    Hadera, 3810101
    Israel

    Active - Recruiting

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