Collecting and Storing Tissue From Young Patients With Cancer

  • participants needed
  • sponsor
    Children's Oncology Group
Updated on 4 October 2022
myeloid leukemia
lymphoid leukemia
bone marrow procedure
lymphocytic leukemia
primary cancer
brain tumor
germ cell tumor
cytology specimen collection procedure
germ cell tumors
rhabdoid tumor
acute lymphoblastic leukemia (all)
ewing sarcoma/peripheral primitive neuroectodermal tumor
ewing sarcoma family of tumors


This laboratory study is collecting and storing tissue, blood, and bone marrow samples from young patients with cancer. Collecting and storing samples of tissue, blood, and bone marrow from patients with cancer to study in the laboratory may help doctors learn more about changes that may occur in DNA and identify biomarkers related to cancer.



I. Establish and bank cell lines and/or xenografts from pediatric patients with cancer.

II. Establish continuous cell lines, under carefully controlled conditions, from pediatric patients with cancer.

III. Establish transplantable xenografts in immunocompromised mice from tumor cells that are difficult to establish as continuous cell lines in vitro.

IV. Create a bank of cell lines and generate sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols. V. Characterize cell lines from childhood cancers with respect to DNA short tandem repeat molecular profile as a "fingerprint" of original cell line identity.

VI. Characterize cell lines for the ability for sustained growth in tissue culture and/or as mouse xenografts.

VII. Characterize cell lines for mycoplasma contamination. VIII. Characterize cell lines for expression of molecular makers that confirm the tumor-type of the cell line and the immortal nature of the cells (telomerase) and the expression of molecular markers that may correlate with drug resistance.

OUTLINE: This is a multicenter study.

Specimens are stratified according to disease (acute lymphoblastic leukemia vs acute myeloid leukemia vs lymphoma vs osteogenic sarcoma vs Ewing family of tumors vs rhabdomyosarcoma vs primitive neuroectodermal tumor vs glioma vs astrocytoma vs rhabdoid tumors vs hepatoblastoma vs retinoblastoma vs Wilms tumor vs germ cell tumors vs other diagnoses).

Leftover tissue from diagnostic procedures and/or surgery is cryopreserved and banked. Blood and/or bone marrow are also collected and banked. Cell lines are established and characterized via reverse-transcriptase polymerase chain reaction and/or flow cytometry for biomarkers and by DNA fingerprinting. Markers to be identified may include the following:

NEUROBLASTOMA: tyrosine hydroxylase, protein gene product (PGP) 9.5, GD2, HLA class I, and HSAN 1.2 antigens


RETINOBLASTOMA: interphotoreceptor retinoid-binding protein



ALL CELL TYPES: telomerase expression including hTR and hTERTMutations of TP53 gene are detected by flow cytometry and/or immunocytochemistry.

No results of these tests are provided to the patient, the patient's physician, or the patient's medical records.

Condition Acute Lymphoblastic Leukemia, Acute Myeloid Leukemia, Central Nervous System Neoplasm, Ewing Sarcoma, Germ Cell Tumor, Leukemia, Lymphoma, Malignant Neoplasm, Neuroblastoma, Osteosarcoma, Retinoblastoma, Rhabdoid Tumor, Rhabdomyosarcoma, Soft Tissue Sarcoma
Treatment laboratory biomarker analysis, flow cytometry, biologic sample preservation procedure, cytology specimen collection procedure, DNA analysis, reverse transcriptase-polymerase chain reaction
Clinical Study IdentifierNCT00898755
SponsorChildren's Oncology Group
Last Modified on4 October 2022


Yes No Not Sure

Inclusion Criteria

All malignant tissues from childhood cancers allowed including the following
Brain tumors (all types)
Tissue should be submitted to CNS Committee Resource labs to be forwarded for this study, unless instructed otherwise on the COG web site
Ewing family of tumors
Other soft tissue sarcomas
Osteogenic sarcomas
Rhabdoid tumors
Viable material for cell culture for neuroblastoma is collected via COG-ANBL00B1 and should not be submitted via this study unless the patient cannot be enrolled on COG-ANBL00B1
Anaplastic Wilms tumor
Germ cell tumors
Acute myeloid leukemia (AML)
Blood samples and bone marrow samples from patients at second relapse and beyond may be submitted for this study
Bone marrow samples at diagnosis or first relapse must be submitted to an AML resource lab and will be forwarded for this study at the discretion of the AML Committee
Acute lymphoblastic leukemia (ALL)
Blood samples may be submitted directly to this study
Bone marrow samples must be submitted to an ALL resource lab and will be forwarded for this study at the discretion of the ALL Committee
Enrolled on a COG therapeutic, biology, or tissue banking protocol that allows
Participation in this protocol is not permitted until after tissue requirements for any active COG disease-specific therapeutic, biology, or banking protocols have been satisfied
collection of tissue for research and submission to a COG-designated resource
Material may only be submitted for this protocol if tissue is available in excess of that required for satisfying active disease-specific therapeutic and biological protocols
Patients with diagnosis pending are eligible
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