Intra-Lymphatic STI-3031 Using the Sofusa DoseConnect Device for the Treatment of In-Transit Melanoma, The Sofusa-2 Study

  • End date
    Jun 1, 2024
  • participants needed
  • sponsor
    Mayo Clinic
Updated on 30 June 2022
ct scan
platelet count
metastatic melanoma
anticoagulant therapy
neutrophil count
tumor cells


This phase Ib trial tests the safety, side effects, and best dose of STI-3031 given directly into the into the lymph nodes or the lymph vessels (intra-lymphatic) using the Sofusa DoseConnect device in treating patients with melanoma that has spread through a lymph vessel and begins to grow more than 2 centimeters away from the primary tumor but before it reaches the nearest lymph node (in-transit). Immunotherapy with monoclonal antibodies, such as STI-3031, may help the body's immune system attack the cancer, and may interfere with the ability of tumor cells to grow and spread.



I. To determine the feasibility, safety, and maximum tolerated dose (MTD) of anti-PD-L1 monoclonal antibody IMC-001 (STI-3031) that can be administered through the DoseConnect device in patients with metastatic melanoma demonstrating in-transit and/or regional lymph node metastases.


I. To assess clinical benefit rates to STI-3031 administered via DoseConnect device in patients with melanoma in-transit and/or reginal lymph node metastases.


I. To assess lymphatic drainage of the affected extremity via intralymphatic indocyanine green (ICG) prior to first treatment cycle and prior to last treatment cycle.

II. To assess for changes in antitumor immunity as a result of therapy.


Patients receive STI-3031 intra-lymphatically via the DoseConnect device over 1-8 hours once weekly (QW) on days 1, 8, 15, 22, 29, and 36 of cycle 1, and once every 2 weeks (Q2W) on days 1, 15, and 29 of cycle 2. Treatment repeats every 42 days for up to 2 cycles in the absence of disease progression or unacceptable toxicity. Patients with complete response (CR) at the end of cycle 2 receive 1-2 additional cycles in the absence of disease progression or unacceptable toxicity. Patients with partial response (PR) or stable disease (SD) at the end of cycle 2 continue treatment for a total of 9 cycles in the absence of disease progression or unacceptable toxicity.

After completion of study treatment, patients are followed at 2-4 weeks, 90 days and every 3 months for up to 2 years after study enrollment.

Condition Clinical Stage III Cutaneous Melanoma AJCC v8, Melanoma, Metastatic Melanoma, Recurrent Metastatic Melanoma
Treatment Punch biopsy, Anti-PD-L1 Monoclonal Antibody IMC-001
Clinical Study IdentifierNCT05393713
SponsorMayo Clinic
Last Modified on30 June 2022


Yes No Not Sure

Inclusion Criteria

Age >= 18 years
Disease characteristics
Newly diagnosed, recurrent, or previously treated in-transit metastatic melanoma (ITM) confined to a single limb with or without regional lymph node involvement
For patients with ITM, one of the following must be true
A visible superficial ITM, non-nodal lesion with longest diameter >= 1.0 cm in diameter as assessed using a ruler (e.g., skin nodules) NOTE: Documentation by color photography, including a ruler is required OR
A malignant regional lymph node with short axis > 1.0 cm as assessed by computed tomography (CT) scan (CT scan slice thickness recommended to be no greater than 5 mm) OR
A non-visible, non-nodal soft tissue mass of the involved extremity with longest diameter >= 1.0 cm as measured with CT scan, CT component of a positron emission tomography (PET)/CT, or magnetic resonance imaging (MRI)
Newly diagnosed, recurrent or previously treated metastatic melanoma of the lymph
nodes in lymphatic beds accessible to limb-lymphatic infusion (Example: lower
limb lymphatic accessible femoral, inguinal pelvic and/or retroperitoneal
lymph node metastases; upper limb: axillary, infraclavicular and/or subclavian
lymph node metastases)
For patients with lymph node metastases only (non ITM) the following must be
At least one tumor involved lymph nodes must be >= 15 mm as assessed by CT, PET or MRI (target lesion by Response Evaluation Criteria in Solid Tumors [RECIST])
Hemoglobin >= 8.0 g/dL (obtained =< 15 days prior to registration)
Absolute neutrophil count (ANC) >= 1500/mm^3 (obtained =< 15 days prior to registration)
Platelet count >= 75,000/mm^3 (obtained =< 15 days prior to registration)
Total bilirubin =< 1.5 x upper limit of normal (ULN) (obtained =< 15 days prior to registration)
Alanine aminotransferase (ALT) and aspartate transaminase (AST) =< 3.0 x ULN (obtained =< 15 days prior to registration)
Serum creatinine =< 2.0 × ULN (obtained =< 15 days prior to registration)
Calculated creatinine clearance >= 40 ml/min using the Cockcroft-Gault formula (obtained =< 15 days prior to registration)
Prothrombin time (PT)/international normalized ratio (INR)/partial thromboplastin time (PTT, aPTT) PT/INR/aPTT =< 1.5 × ULN or if patient is receiving anticoagulant therapy INR or aPTT is within target range of therapy (Within 15 days prior to registration)
Eastern Cooperative Oncology Group (ECOG) performance status (PS) 0 or 1
Negative pregnancy test done =< 7 days prior to registration, for persons of childbearing potential only
Persons able to become pregnant or able to father a child must be willing to use an adequate method of contraception while on treatment and for 180 days (6 months) after last treatment dose on this study
Provide written informed consent
Rochester only: Willingness to provide mandatory blood specimens for correlative research
Willingness to provide mandatory tissue specimens for correlative research
Willing to return to enrolling institution for 3-month follow-up (during the Active Monitoring Phase of the study)

Exclusion Criteria

Any of the following because this study involves an investigational agent whose genotoxic, mutagenic, and teratogenic effects on the developing fetus and newborn are
Pregnant persons
Nursing persons
Persons of childbearing potential who are unwilling to employ adequate contraception
Persons expecting to conceive or father children during the study or within 180 days (6 months) after the last treatment on this study
Metastatic melanoma beyond in-transit metastases (ITM) and regional lymph nodes (LNs)
that cannot be accessed by intralymphatic infusion by DoseConnect (example
visceral or active central nervous system [CNS] metastatic disease)
ITM involving the hands and feet (not accessible to DoseConnect infusion)
ITM NOT involving a limb (i.e., head, neck, or trunk)
Prior radiation of ITM that are being evaluated as measurable lesions
Any of the following prior therapies
Allogeneic hematopoietic stem cell transplantation (HSCT)
Solid organ transplantation
Co-morbid systemic illnesses or other severe concurrent disease which, in the judgment
of the investigator, would make the patient inappropriate for entry into this
study or interfere significantly with the proper assessment of safety and
toxicity of the prescribed regimens
Immunocompromised patients and patients known to be human immunodeficiency virus (HIV) positive and currently receiving antiretroviral therapy
NOTE: Patients known to be HIV positive, but without clinical evidence of an immunocompromised state, are eligible for this trial
Active autoimmune disease requiring systemic treatment < 2 years prior to
registration, documented history of severe autoimmune disease, or a syndrome
that requires systemic steroids or immunosuppressive agents with use of
disease modifying agents, corticosteroids, or immunosuppressive drugs
NOTE: Exceptions are allowed for the following conditions
Resolved childhood asthma/atopy
Intermittent use of bronchodilators or inhaled steroids
Daily steroids at dose of =< 10mg of prednisone (or equivalent)
Local steroid injections
Stable hypothyroidism on replacement therapy
Stable diabetes mellitus on therapy (with or without insulin)
Sjogren's syndrome
Replacement therapy (e.g., thyroxine, insulin, or physiologic corticosteroid replacement therapy for adrenal or pituitary insufficiency, etc.) which is not considered a form of systemic treatment and is allowed
Uncontrolled intercurrent illness including, but not limited to
Ongoing or active infection requiring systemic therapy
Interstitial lung disease
Serious, chronic gastrointestinal conditions associated with diarrhea (e.g., Crohn's disease or others)
Known history of hepatitis B (i.e., known positive hepatitis B virus [HBV] surface antigen [HBsAg] reactive)
Known active hepatitis C (i.e., positive for hepatitis C virus [HCV] ribonucleic acid [RNA] detected by polymerase chain reaction [PCR])
Known active tuberculosis (TB)
Symptomatic congestive heart failure
Unstable angina pectoris
Unstable cardiac arrhythmia or
Psychiatric illness/social situations that would limit compliance with study requirements (e.g., known substance abuse)
Receiving any other investigational agent which would be considered as a treatment for the primary neoplasm
History of severe hypersensitivity reactions to other monoclonal antibodies or known hypersensitivity to the study intervention or its excipients, indocyanine green (ICG) dye or iodine
Prior history of Grade 4 immune related adverse event (irAE) with prior immune checkpoint inhibitor (ICI) therapy or failure to recover (< Grade 1) from immune-related adverse event(s) from prior ICI therapy
Failure to recover from any adverse events related to any of the following therapies received prior to registration
Targeted therapies (e.g., dabrafenib)
Other investigational agents
Radiation therapy
Minor surgical or interventional procedure (NOTE: Biopsy of same limb for diagnosis is allowed)
Major surgical procedure
Previously untreated metastatic melanoma (visceral or CNS). NOTE: Patients with previously treated systemic and CNS melanoma who have been free of disease at systemic sites for >= 12 weeks are eligible for treatment in this study
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