The Effect of Sweet Orange and Pomegranate Extract Supplementation on Exercise Capacity in Middle-aged Adults

  • End date
    Dec 31, 2022
  • participants needed
  • sponsor
    Maastricht University
Updated on 1 December 2021
Accepts healthy volunteers


The aim of the present study is to investigate the effect of 12-week long daily administration of a sweet orange and pomegranate extract on exercise capacity in healthy adults.


Background of the study: Polyphenols have been studied for their protective effect against the development of ROS-related diseases like cancers, cardiovascular diseases, diabetes, osteoporosis, and neurodegenerative diseases. The combined supplementation of pomegranate and sweet orange polyphenols could be an effective strategy to improve exercise performance, due to their antioxidant character and ability to stimulate NO production, to stimulate mitochondrial biogenesis and to accelerate muscle repair and decrease muscle tissue damage. The study aims to assess the effect of a combined supplementation of pomegranate and sweet orange extract on exercise capacity, physical activity, muscle strength and quality of life in healthy adults.

Objective of the study: The aim of the present study is to investigate the effect of 12-week long daily administration of a sweet orange and pomegranate extract on exercise capacity in healthy adults (40 - 65 y).

Study design: Randomized, double-blind, placebo-controlled parallel trial

Study population: Healthy, overweight, sedentary adults between 40 and 65 years old.

Intervention: 12-week supplementation with 650 mg sweet orange and pomegranate extract (2 capsules per day) compared to placebo (760 mg maltodextrin).

Primary outcome of the study: Change in aerobic capacity (VO2max), assessed with an Ekblom-Bak submaximal cycling test.

Secondary study parameters/outcome of the study:

  • Daily physical activity as determined by accelerometer
  • Dietary intake as measured by a 3-day food record
  • Handgrip strength
  • Muscle cell proliferation and differentiation and mitochondrial biogenesis assessed in skeletal muscle biopsies
  • Quality of Life as measured by the WHO-QOL-100 questionnaire
  • General health as measured by SF-36 questionnaire
  • Vitality as measured by the Dutch Vitality Questionnaire (Vita-16)
  • Cardiometabolic health biomarkers, cortisol, C-reactive protein determined with ELISA
  • Antioxidative capacity measured with a Trolox equivalent antioxidant capacity (TEAC) assay

Condition Exercise Capacity
Treatment Sweet orange and pomegranate extract, Maltodextrin control
Clinical Study IdentifierNCT05133778
SponsorMaastricht University
Last Modified on1 December 2021


Yes No Not Sure

Inclusion Criteria

Healthy male/female (based on medical history provided during a general health questionnaire)
Age between 40 - 65 yrs
Overweight (BMI 25 to 29.9 kg/m2)
Sedentary subjects (Low Level [<600 MET-minutes/week] as assessed by the Physical Activity Questionnaire (IPAQ))

Exclusion Criteria

Allergy to test product/control or citrus fruits and pomegranate
Medical conditions that might interfere with endpoints or compromise participant safety during testing (e.g. Cardiovascular diseases, cancer, Parkinson's disease, Gastrointestinal diseases or abdominal surgery) to be decided by the principal investigator
Use of medication that might interfere with endpoints (i.e.: -blockers, antioxidant, antidepressants)
High fasting blood glucose (FBG 100 mg/dL)
Recent skeletal muscle injury in less than one month before the start of the study
Use of antibiotics within 3 months prior to Visit 2
Use of probiotics or supplements containing vitamins, minerals or antioxidants four weeks prior to Visit 2
Regular smoking (including use of e-cigarettes)
Inability to correctly perform the PA test during screening/familiarization
Abuse of alcohol (alcohol consumption >20 units/week) and/or drugs
Plans to change diet or medication for the duration of the study
Treatment with an investigational drug (phase 1-3) 180 days before the start of the study
Inability to understand study information and/or communicate with staff
Clear my responses

How to participate?

Step 1 Connect with a study center
What happens next?
  • You can expect the study team to contact you via email or phone in the next few days.
  • Sign up as volunteer to help accelerate the development of new treatments and to get notified about similar trials.

You are contacting

Investigator Avatar

Primary Contact



Additional screening procedures may be conducted by the study team before you can be confirmed eligible to participate.

Learn more

If you are confirmed eligible after full screening, you will be required to understand and sign the informed consent if you decide to enroll in the study. Once enrolled you may be asked to make scheduled visits over a period of time.

Learn more

Complete your scheduled study participation activities and then you are done. You may receive summary of study results if provided by the sponsor.

Learn more

Similar trials to consider


Browse trials for

Not finding what you're looking for?

Every year hundreds of thousands of volunteers step forward to participate in research. Sign up as a volunteer and receive email notifications when clinical trials are posted in the medical category of interest to you.

Sign up as volunteer

user name

Added by • 



Reply by • Private

Lorem ipsum dolor sit amet consectetur, adipisicing elit. Ipsa vel nobis alias. Quae eveniet velit voluptate quo doloribus maxime et dicta in sequi, corporis quod. Ea, dolor eius? Dolore, vel!

  The passcode will expire in None.

No annotations made yet

Add a private note
  • abc Select a piece of text from the left.
  • Add notes visible only to you.
  • Send it to people through a passcode protected link.
Add a private note