Sperm Oxidative Stress and Pregnancy Success During IUI and IVF

  • STATUS
    Recruiting
  • End date
    Jun 1, 2023
  • participants needed
    250
  • sponsor
    Fertilys
Updated on 28 August 2021

Summary

Oxidative stress (OS) is characterized by an imbalance between the production of reactive oxygen species (ROS) and the ability of the body to eliminate them. Such an imbalance can lead to lipid peroxidation, DNA damage and cell apoptosis. Studies have suggested that infertile men are more likely to have high concentrations of ROS in their seminal plasma. The investigators hypothesize that a high level of oxidative stress (OS) in patients with abnormal sperm parameters could influence fertilization and / or pregnancy success. Our first goal is to compare OS levels to semen parameters, as defined by the World Health Organization in 2010, as well as DNA fragmentation and chromatin decondensation in sperm. Our second objective is to compare OS levels in sperm to fertilization and blastulation rates at first, and then measure the impact of OS levels on pregnancy success, both in intrauterine insemination (IUI) and in vitro fertilization (IVF). Sperm samples from men in 3 groups will be analyzed: a group of fertile men with confirmed paternity (n = 50), a group of men in IVF (n = 100) and a group of men in their first three cycles of IUI (n = 100). The static oxidation reduction index (sORP) will be measured by the MiOXSYS system, a rapid sperm analysis system using electrochemical technology. A small portion of the fresh sperm sample will be deposited on a MiOXSYS sensor and the ORP will be measured. Sperm ROS will also be measured using the CellROX Deep Red probe. The sperm parameters will be measured by the techniques used routinely in the Fertilys Reproductive Health Center andrology lab. Regarding couples in IVF, fertilization and blastulation rates, embryo quality and pregnancy success will be noted. Pregnancy success will be noted for IUI couples. If the usefulness of sORP levels in predicting pregnancy and its outcome is demonstrated, it could be a new marker in the diagnosis of male infertility and act as a guide for clinicians to apply appropriate treatment.

Details
Condition Stress, Oxidative Stress, Physiological Stress
Clinical Study IdentifierNCT03539874
SponsorFertilys
Last Modified on28 August 2021

Eligibility

Yes No Not Sure

Inclusion Criteria

All men with confirmed paternity aged 18 to 40 years, agreeing to participate in the study and having signed the consent form
All patients aged 18 to 40 using IVF or their first cycle of IUI at the Fertilys Reproductive Health Center, agreeing to participate in the study and having signed the consent form

Exclusion Criteria

Patients under 18 and over 40 years old
Men who can not produce a sperm sample by anterograde ejaculation
Men going to the Fertilys Reproductive Health Center for a post-vasectomy spermogram
Couples who have already completed IUI cycles
Couples using gamete donation (eggs or sperm)
Men with a mobile sperm concentration of less than 5 millions/mL after preparation of the sperm sample during the IUI process
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