Pathogens Responsible for Respiratory Infections Associated With Drowning in Seawater (APIR-NOY)

  • STATUS
    Recruiting
  • End date
    Sep 15, 2023
  • participants needed
    15
  • sponsor
    Centre Hospitalier Universitaire de Nice
Updated on 28 May 2022
pneumonia
resuscitation

Summary

Little data is currently available on the type of pathogen responsible for respiratory infections following drowning. Many environmental germs present in seawater are described as difficult to culture on standard media but are potentially pathogenic. Even using specific culture media nearly 90% of the bacteria present in the water remain non-cultivable. The use of 16S and 18S rDNA amplification followed by high throughput sequencing on respiratory samples could allow us to objectify these bacteria potentially involved in the physiopathological process secondary to drowning and thus improve their overall management.

Carrying out a bronchoalveolar lavage (BAL) within 24 hours after admission to intensive care with analysis on standard and specific culture media of environmental germs, carrying out antibiograms, DNA extraction, amplification by universal 16 and 18S rDNA primers followed by high flow sequencing and phylogenetic analysis of pathogens found. Comparison of these results with the same analyses performed on seawater samples taken near the drowning site and during a new AML at 72h for patients still intubated and showing signs of pneumonia in order to determine if the pathogens detected at the entrance persist and are present in the environment.

AML at the patient's entrance, on day 3 and environmental sampling in the drowning environment within 24 hours. Standard and specific bacteriological analyses with molecular biology techniques (amplification 16 and 18S rDNA) carried out at Pr RUIMY's laboratory (Nice University Hospital) sequencing on INRA's Toulouse genomics platform. Phylogenetic data capture and analysis at IRCAN (Bioinformatics, Croce Olivier). Patient follow-up until resuscitation discharge.

Details
Condition Drowning
Treatment bronchoalveolar lavage
Clinical Study IdentifierNCT03498963
SponsorCentre Hospitalier Universitaire de Nice
Last Modified on28 May 2022

Eligibility

Yes No Not Sure

Inclusion Criteria

adult patient
hospitalized in intensive care for severe drowning in sea water (Spilzman stage 5 or 6) (4)
intubated within 24 hours of admission or before admission to intensive care
affiliated to social security

Exclusion Criteria

patients under guardianship, trusteeship or detention
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